Rapid advances in molecular biology have accelerated the production of a great number of protein-based therapeutic agents. The major cost in producing these proteins appears to be associated with their purification from the complex mixture of the crude extract. A major challenge to the protein biochemist and the biochemical engineer is the development of rapid, efficient, and cost-effective purification systems. This volume presents state-of-the-art reviews of current methods used in the purifica tion of biological macromolecules that are based on molecular interactions. Thus, the major emphasis is placed on affinity-related techniques. Part I provides a general introduction to affinity chromatography and includes a chapter describing an interesting new technique called "slalom chromatography" for DNA fractionations. Affinity chromatography using molecules of biological origin as the affinity ligand is covered in Part II. Part III describes the use of a special class of biomolecules, antibodies, as affinity ligands. Affinity chromatography with biomimetic ligands is discussed in Part IV. Newer concepts and their applications in bioseparation are presented in Part V. Part VI covers affinity-related techniques such as affinity-based extracorporeal shunts, affinity electrophoresis, affinity precipitation, and affinity extraction. I would like to express my sincere thanks to all the authors, who are recognized experts in their respective fields, for their cooperation and contributions. I thank the editorial staff of Plenum Press for their professionalism, and Mary Phillips Born, Senior Editor, for her encouragement. The support of my family (Ping and Peilin) made it possible to complete editing this book. That T.
Auflage
Softcover reprint of the original 1st ed. 1993
Sprache
Verlagsort
Zielgruppe
Für Beruf und Forschung
Research
Illustrationen
Maße
Höhe: 235 mm
Breite: 155 mm
Dicke: 32 mm
Gewicht
ISBN-13
978-1-4899-1874-1 (9781489918741)
DOI
10.1007/978-1-4899-1872-7
Schweitzer Klassifikation
I: General Introduction.- 1. Affinity Chromatography: An Overview.- 2. Weak-Affinity Chromatography.- 3. Affinity Chromatography on Inorganic Support Materials.- 4. 2-Fluoro-1-methylpyridinium (FMP) Salt-Activated Gels: Properties and Uses in Affinity Chromatography and Enzyme Immobilization for Analytical Applications.- 5. Slalom Chromatography: A Size-Dependent Separation Method for DNA Molecules Based on a Hydrodynamic Principle.- II: Affinity Chromatography with Biological Ligands.- 6. Applications of Bacterial Immunoglobulin-Binding Proteins to the Purification of Immunoglobulins.- 7. Affinity Chromatography of Oligosaccharides and Glycopeptides with Immobilized Lectins.- 8. Selective Isolation of C-Terminal Peptides by Affinity Chromatography.- 9. Receptor-Affinity Chromatography (RAC).- 10. Immobilized Artificial Membrane Chromatography: Prediction of Drug Transport across Biological Barriers.- 11. Affinity Chromatography Using Immobilized Antisense-Family Peptides.- 12. Chromatographic Resolution of Chiral Compounds by Means of Immobilized Proteins.- 13. Chromatography with Cyclodextrin-Based Stationary Phases.- III: Immunoaffinity Separation.- 14. Suitable Antibodies as Ligands in Affinity Chromatography of Biomolecules.- 15. Immunoaffinity Purification of Organelles.- 16. Immunoaffinity Separation of Cells Using Monosized Magnetic Polymer Beads.- IV: Affinity Chromatography with Biomimetic Ligands.- 17. Affinity Chromatography with Immobilized Dyes.- 18. Pseudo-Biospecific Affinity Ligand Chromatography: The Case of Immobilized Histidine as a Universal Ligand.- 19. Synthetic Protein Surface Domains as Bioactive Stationary Phases: Metal Ion-Dependent Macromolecular Recognition and Biospecific Metal Ion Transfer.- 20. Affinity Chromatography with Immobilized Benzeneboronates.- 21. Affinity Chromatographic Removal of Pyrogens.- 22. Molecular Interactions in Hydrophobic Chromatography.- V: Novel Concepts and Applications.- 23. Biorecognition in Molecularly Imprinted Polymers: Concept, Chemistry, and Application.- 24. Bioseparation and Catalysis in Molecularly Imprinted Polymers.- 25. Use of Heterobifunctional Ligands in Affinity Chromatographic Processes.- 26. Covalent Chromatography.- 27. Aza-Arenophilic Interaction: Novel Mode of Protein Adsorption and Applications in Immunoglobulin Purification.- VI: Affinity-Related Techniques.- 28. Model Systems Employing Affinity Chromatography for Extraction of Toxic Substances Directly from Whole Blood.- 29. Applications of Affinity Binding to the Development of Heparin Removing and Sensing Devices.- 30. Affinity Precipitation.- 31. Affinity Separation of Nucleic Acids on Monosized Magnetic Beads.- 32. Affinity Ultrafiltration for Protein Purification.- 33. Affinity Partitioning of Biomolecules in Aqueous TVvo-Phase Systems.- 34. Affinity Electrophoresis of Macromolecules: General Principles and Their Application to Nucleic Acids.- 35. Reversed Micelles for Protein Purification.
