During the past decade we have witnessed a revolution in analytical methods. The development of vapor phase chromatography for the separation and analysis of classes of substances ranging from metals and gases to a wide variety of organic materials has been one of the most exciting of these new techniques. Gas-liquid chromatography for the measurement of steroids is particularly significant for endocrinologists and reports during the past several years have demonstrated its usefulness. Because of the growing interest in this method, a committee of the Endocrinology Study Section composed of Drs. R. M. Dodson, Seymour Lieberman, Hilton A. Salhanick, and Ralph E. Peterson, felt that the time was propitious to hold this Workshop and it is on their behalf that I welcome you. We hope to obtain enough data during these sessions so that those attending this conference and those who may read the proceedings will be able to make an informed judgement about the usefulness of gas-liquid chromatography for the analysis of steroids in biological fluids.
Thus, I hope that there will be adequate documentation of the reliability of the methods as well as a comparison of the advantages and disadvantages of this analytical method with other classical methods. If we can do this, this Workshop will provide a significant base of practical considerations about gas chromatographic analytic techniques. I would like to thank Drs. T. F. Gallagher, H. Wilson, H. Salhanick and L. Engel for agreeing to serve as Chairmen of the sessions.
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Springer Science+Business Media
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ISBN-13
978-0-306-30204-6 (9780306302046)
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Schweitzer Klassifikation
Session I: Ketosteroids and Testosterone.- The Specificity of a Gas Chromatographic Method for the Determination of Urinary Androsterone, Etiocholanolone and Dehydroeplandrosterone in Urine.- The Indirect Analysis of Urinary 17-Oxygenated C19 Steroid Sulphates by Gas-Liquid Chromatography.- A Technic for Fractionation of 17-Ketosteroids from Urine.- Gas Chromatographic Method for the Determination of Testosterone in Human Urine.- Estimation of Testosterone in Blood by Gas-Liquid Chromatography.- The Estimation of Plasma Androgens by Gas-Liquid Chromatography.- Discussion.- Session II: Corticoids.- The Estimation of Unconjugated Cortisol Metabolites in Urine by Gas Chromatography.- Gas Chromatography of Cortols and Cortolones.- Urinary Corticoids by Gas Chromatography.- A Specific Method for the Gas Chromatographic Determination of Aldosterone and Adrenocortical Steroids: Application to Adrenal Venous Blood of Vertebrates.- Analysis of Aldosterone in Urine by Double Isotope Dilution and Gas-Liquid Chromatography.- Discussion.- Session III: Progesterone and C21-Diols and Triols.- Measurement of Urinary Steroids by Gas Chromatography. 3?, 17-Dihydroxypregnane-20-One and C21 Triols.- Analysis of Urinary Pregnanediol and Pregnanetriol by Gas-Liquid Chromatography.- The Application of Gas-Liquid Chromatography to the Measurement of Plasma Progesterone.- Determination of Progesterone in Human Peripheral Blood Using Gas-Liquid Chromatography with Electron Capture Detection.- The Analysis of Plasma Progestins by Gas Chromatography.- Discussion.- Session IV: Estrogens.- Gas Chromatography and Its Role in the Versatile Analysis of Urinary Estrogens.- Determination of Urinary Estrogens by Gas Chromatography.- Gas Chromatography of Estriol in Pregnancy Urine.- Analysis of Estrogens in the Urine of Non-Pregnant Women by Gas-Liquid Chromatography.- The Measurement of Estriol in Urine and Amniotic Fluid by Gas-Liquid Chromatography.- Gas Chromatography of Free and Conjugated Estrogens in Blood Plasma.- Estimation of Estradiol-17? by Gas-Liquid Chromatography with Electron Capture Detection.- Gas Chromatography of Estrogens and Other Steroids from Endocrine Tissues.- Discussion.- Session V: Some Theoretical and Practical Aspects of Gas-Liquid Chromatography.- Some Aspects of the Chemistry of Gas-Liquid Chromatography.- Discussion.- Some Practical Problems in Gas-Liquid Chromatography.- Comments on Techniques on Gas Chromatography.- Discussion.- The Utility of GLC for Analyzing Specific Steroids in Biological Fluids.
