PART B: Analysis of Polynucleotides, Nucleotides, Nucleosides, and Bases. Isolation and Extraction of Nucleotides from Tissues Prior to HPLC. Purification of Plant Nucleotide Extracts Prior to HPLC Analysis. Optimization and Prediction of Retention of Nucleic Acid Components Separated by HPLC. Separation of Nucleobases and Nucleosides on Silica Gel. The Liquid Chromatographic Behavior of Outer-Sphere and Inner-Sphere Complexes of Nucleosides and Nucleotides with Metal Ions. Separations of Nucleic Acid Components by Bidimensional HPLC. Simultaneous Analysis of Nucleotides, Nucleosides, and Bases by Ion Exchange and On-Line Exchange-Reversed Phase Chromatography. Analysis of Urinary and Plasma Nucleosides by Column Switching Technique. Labeling of Pyrimidine Compounds for Fluorescent Detection in HPLC. Continuous Flow UV-VIS Scanning of Nucleic Acid Components Separated by HPLC. Planar Chromatography and Its Applications to Nucleic-Acid Research. Miscellaneous Applications. HPLC of Pharmacologically Important Nucleobase and Nucleoside Analogs: 6-Thioguanine, 5-Fluorouracil, and Cytarabine. HPLC Investigation of PT (III) Coordination Complexes with Nucleic Acid Components. Index.