This handbook provides a comprehensive review of the most current developments in nucleic acid research by leading scientists worldwide. Chapters focus on structure elucidation methods, fundamentals of HPLC; analysis of nucleic acids and oligonucleotides; analysis of polynucleotides, nucleotides, nucleosides and bases; nucleic acid components in biological fluids and their diagnostic value; and miscellaneous application. The format is organized according to chromatographic techniques and by chemical classifications. Each subject is presented in the form of a critical review.
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978-0-8493-3020-9 (9780849330209)
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Schweitzer Klassifikation
PART A: Structure Elucidation of Nucleic Acid Components. Fundamentals of HPLC. Analysis of Nucleic Acids and Oligonucleotides. Extraction of Nucleic Acids from Tissues. Gel Filtration Chromatography of RNAs and DNS Fragments. Separation of tRNAs and Oligonucleotides by Mixed Mode Chromatography. Anion-Exchange and Reversed-Phase HPLC of Synthetic Oligonucleotides. Nucleic Acid Components in Biological Fluids. RPLC Separation of RNA and DNA Hydrolysates. Nucleotides in Tissue Extracts. Determination of Adenine Nucleotides and Creatine Phosphate in Various Mammalian Tissues. HPLC Determination of "Unusual Nucleotides" (bis[5*-nucleosidyl] oligophosphates and guanosine 5*, 3*-bispolyphosphates)I in Cellular Extracts. Nucleosides and Bases in Extracellular Fluids Analyzed by HPLC. Nucleic Acid Components and Related Compounds in Uremic Hemodialysates Analyzed by HPLC. Screening for Inborn Errors of Purine and Pyrimidine Metabolism by Bidimensional TLC and HPLC. Anion-Exchange Analysis of Isotopically Labeled Nucleotides, Nucleosides, and Bases in Metabolic Disorders. HPLC Determination of Enzyme Activities of Nucleotide/Nucleoside Metabolism. Index.
