This text offers a concise background in HPLC methodology and includes illustrative examples of some of the key advances made in the receptor field through application of this chromatographic technique. A brief historical overview puts into perspective the specific state-of-the-art of HPLC laboratory applications that are contained in the book's chapters. Technical contributions discuss how best to select the HPLC mode for a particular biochemical problem, including the most effective use of a particularly sensitive technique, microbore HPLC for synthesis, purification and characterization of synthetic peptides. The text will benefit biochemists and molecular biologists.
Reihe
Sprache
Verlagsort
Verlagsgruppe
Zielgruppe
Für höhere Schule und Studium
Für Beruf und Forschung
Illustrationen
Maße
Höhe: 261 mm
Breite: 184 mm
Gewicht
ISBN-13
978-0-471-56231-3 (9780471562313)
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Schweitzer Klassifikation
The isolation of endogenous peptides, with an emphasis on their biologic activity; the application of HPLC to receptor mapping (illustrated with case studies of the structural features of receptors discovered with this technique); the use of immunoaffinity chromatography to recover biologically active proteins; the application of a wide range of HPLC modes to the study of steroid hormone receptors; a highly detailed strategy for isolating small amounts of proteins and peptides for microsequence analysis.
