Is noninvasive, risk-free prenatal diagnosis of fetal genetic characteristics still a fantasy, or will it soon become reality? The current status of both the leading European research groups as well as the NIH-funded NIFTY study are reported here, indicating that certain fetal genetic traits can now be examined efficiently in a noninvasive manner. Considerable focus is placed on new laser-mediated systems for the effective micro-manipulation of single fetal cells, as well as their analysis by single-cell PCR and the pitfalls to avoid when performing such analyses. Other issues addressed in depth include: novel enrichment techniques, optimal fetal cell recognition, fetal cell culture, as well as the exciting finding that fetal cell traffic is elevated in certain pregnancy-related disorders, most prominently in preeclampsia. This publication is of interest to researchers in the field, genetic counsellors, gynecologists and obstetricians, and researchers in microchimerism, transplantation and transfusion medicine.
Sprache
Verlagsort
Zielgruppe
Illustrationen
22 figs., 3 in color, 16 tab.
Maße
Höhe: 24 cm
Breite: 17.5 cm
Gewicht
ISBN-13
978-3-8055-7222-4 (9783805572224)
Schweitzer Klassifikation
Circulating DNA in plasma or serum; experience with single cell PCR in preimplantation genetic diagnosis - how to avoid pitfalls; laser-mediated micromanipulation systems - an important tool for the analysis of single cells; why do we need noninvasive prenatal diagnostic procedures?; fetal cells in maternal blood - an overview of the Basel experience; fetal cells in maternal blood at Sheba Medical Center, Israel - analysis of three cell types during the three trimesters of pregnancy; nucleated red blood cell recovery from maternal blood with non-commercial monoclonal antibodies; how to enrich and analyze fetal cells from maternal blood - 5-year experience with PCR, FISH and cultivation; solid phase method for enrichment of fetal cells from maternal blood; fetal nucleated red blood cells in maternal blood during pregnancy - first Czech experience; using AMCA to label the fetal cell antigen in fetal erythroblasts circumvents heme autofluorescence, enhances cFISH efficiency and improves specificity of fetal cell identification; fetal cell detection in maternal blood using the solid phase cytometer ChemScan RDI - preliminary studies; fetal CD34+ cell culture and identification from maternal blood; free fetal DNA in maternal plasma and serum - diagnostic and clinical implications; quantitative analysis of fetal DNA in maternal plasma in preeclamptic pregnancies; circulating trophoblast cells in maternal peripheral blood; transcervical sampling; impact of improved screening efficiency on the future role of non-invasive testing.
