Application of plasmids containing promoters specific for phage-encoded RNA polymerases; Choice and use of cosmid vectors; Genetic approaches to the cloning, modification and characterization of cosmid clones and clone libraries; The purification of eukaryotic polypeptides expressed in escherichia coli; Production and purification of polyclonal antibodies to the foreign segment of B-Galactosidase fusion proteins; Production of monoclonal antibodies against fusion proteins produced in escherichia coli; Expression and secretion of foreign genes in yeast; The use of vectors based on gene amplification for the expression of cloned genes in mammalian cells; Retroviral vectors; The production of transgenic mice by the microinjection of cloned DNA into fetilized one-cell eggs
