Chromatography and Modification of Nucleosides is a four-volume work which provides state-of-the-art chromatography and analytical methods for use in a wide spectrum of nucleic acid modification research. The focus of Part A, is the presentation of advanced methods for modification research on tRNAs, mRNAs, mtRNAs, rRNAs and DNAs. HPLC-UV, GC-MS, NMR, FT-IR and affinity chromatography approaches to nucleic acid modification studies are presented, as are nucleoside, oligonucleotide and nucleic acid isolation techniques. Part B has as its central theme the modified nucleosides of tRNA and the current analytical means for studying tRNA modifications. Modified nucleoside synthesis, function, structural conformation, biological regulation, and occurrence of modification in a wide range of tRNAs are presented, as is a chapter on DNA modification and a chapter on solid phase immunoassay for determining a particular modification. Part C provides the reader with a comprehensive treatment of modified nucleosides as biochemical signals of neoplasia and normal metabolism.
The final volume, Part D, will present structural characterization of unknown nucleosides as well as extensive biochemical, chemical and physical properties of RNA and DNA nucleosides, as a "database" for researchers in the field. Chromatographic methodology will be described for analysis of total modification of tRNAs and DNAs. The chapters are written by leading scientists in their respective fields and present an up-to-date review on the roles of modified nucleosides in nucleic acids which will be extremely useful for workers in chromatography, molecular biology, genetics, biochemistry, biotechnology and the pharmaceutical industry.
Reihe
Sprache
Verlagsort
Verlagsgruppe
Elsevier Science & Technology
Zielgruppe
Für höhere Schule und Studium
Für Beruf und Forschung
Maße
ISBN-13
978-0-444-88598-2 (9780444885982)
Copyright in bibliographic data is held by Nielsen Book Services Limited or its licensors: all rights reserved.
Schweitzer Klassifikation
Contents of Part C. Introduction. Early development of nucleoside markers for cancer (T.P. Waalkes, C.W. Gehrke). 1. Progress and future prospects of modified nucleosides as biological markers of cancer (R.W. Zumwalt et al.). 2. Ribonucleosides in biological fluids by a high-resolution quantitative RPLC-UV method (K.C. Kuo et al.). 3. Ribonucleosides in body fluids: On-line chromatographic cleanup and analysis by a column switching technique (E. Schlimme, K. Siegfried-Boos). 4. High-performance liquid chromatography of free nucleotides, nucleosides, and their bases in biological samples (P.R. Brown, Y.-N. Kim). 5. Isolation and characterization of modified nucleosides from human urines (G.B. Chheda et al.). 6. High performance liquid chromatography of modified nucleosides in human serum (E.P. Mitchell et al.). 7. Modified nucleosides in human blood serum as biochemical signals for neoplasia (F. Salvatore et al.). 8. Biochemical correlations between pseudouridine excretion and neoplasias (F. Cimino et al.). 9. High-performance liquid chromatography analysis of nucleosides and bases in mucosa tissues and urine of gastrointestinal cancer patients (K. Nakano). 10. Modified nucleosides as biochemical markers of asbestos exposure and AIDS (O.K. Sharma, A. Fischbein). 11. RNA catabolites in health and disease (I. Clark et al.). 12. Serum nucleoside chromatography for classification of lung cancer and controls (J.E. McEntire et al.). 13. Modified nucleosides and nucleobases in urine and serum as selective markers for the whole-body turnover of tRNA, rRNA, and mRNA-cap - Future prospects and impact (G. Schoch et al.). Combined Subject Index for Parts A, B and C.
