This book is the first of a two-volume project which attempts to treat the sample handling and detection processes in a liquid chromatographic system in an integrated fashion. The need for more selective and sensitive chromatographic methods to help solve the numerous trace analysis problems in complex samples is undisputed. However, few workers realize the strong interdependence of the various steps - sample handling, separation and detection - which must be considered if one wants to arrive at an optimal solution. By introducing a strong element of selectivity and trace enrichment in the sample preparation step, fewer demands are placed on the quality of the chromatography and often a simple UV detector can be used. By using a selective detection mode, i.e. a reaction detector, the sample handling step can frequently be simplified and more easily automated. The impact of such a ``total system'' approach on handling series of highly complex samples such as environmental specimens or biological fluids can be easily imagined. Each chapter includes sufficient references to the literature to serve as a valuable starting point for more detailed investigation.
Reihe
Sprache
Verlagsort
Verlagsgruppe
Elsevier Science & Technology
Zielgruppe
Für höhere Schule und Studium
Für Beruf und Forschung
Maße
Höhe: 230 mm
Breite: 150 mm
ISBN-13
978-0-444-42881-3 (9780444428813)
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Schweitzer Klassifikation
1. On-line sample handling and trace enrichment in liquid chromatography. The determination of organic compounds in water samples (M.W.F. Nielen et al.). Instrumentation. On-line sample handling and trace enrichment. Miniaturization of pre-column technology. Conclusion and further developments. 2. Determination of drugs and their metabolites in biological samples by fully automated HPLC with on-line solid-liquid extraction and pre-column switching (R. Huber, K. Zech). Sample preparation techniques. Pre-column technique with column switching. Analytical separation system. Applications. 3. Immobilization of compounds for selective interaction with analytes in liquid chromatography (G. Gubitz). Surface modification of siliceous supports. Chemically modified phases for sample pretreatment and selective separation. Chemically modified supports for selective detection. 4. Design and choice of suitable labelling reagents for liquid chromatography (K. Imai, T. Toyo'oka). Derivatization for UV or visible detection. Derivatization for fluorescence detection. Derivatization for electrochemical detection (ED). Derivatization for chemiluminescence (CL) detection. 5. Photodiode array detection and solute recognition in high-performance liquid chromatography (A.F. Fell, B.J. Clark). Digital methods. Development trends. 6. Electrochemical techniques for detection in HPLC (W.Th. Kok). LCEC: State of the art. Amperometric detection. Dual electrode systems. Derivatization for LCEC. Active metal electrodes. On-line coulometric titrations. Electrochemical signal enhancement. 7. Solid-phase reactors in high-performance liquid chromatography (L. Nondek, R.W. Frei). Solid-phase derivatization reactions. The use of tubular reactors for solid-phase derivatizations. Practical applications of SPRs in HPLC. 8. Commercial aspects of post-column reaction detectors for liquid chromatography (R. Weinberger, R.A. Femia). Instrumentation. Applications based systems. How to optimize a post-column chemistry. Prospective. Manufacturers' directory. Subject Index.
