Micromanipulation of Human Gametes and Embryos

This volume is a comprehensive reference on reproductive micromanipulation technology and its applications in in vitro fertilization, genetic diagnosis, animal husbandry, and developmental embryology. The authors give practical guidelines and step-by-step instructions for setting up a micromanipulation laboratory, selecting equipment, fabricating microtools, and performing microsurgical fertilization and related techniques such as assisted hatching in humans. Coverage of preconception and preimplantation genetic diagnosis includes detailed laboratory protocols for biopsy procedures, polymerase chain reaction, and in situ hybridization. The book concludes with a look at the future of emerging techniques such as laser micromanipulation, gene transfer, germ-lined therapy, and nuclear transplantation.
 
This volume is a comprehensive reference on reproductive micromanipulation technology and its applications in in vitro fertilization, genetic diagnosis, animal husbandry, and developmental embryology. The authors give practical guidelines and step-by-step instructions for setting up a micromanipulation laboratory, selecting equipment, fabricating microtools, and performing microsurgical fertilization and related techniques such as assisted hatching in humans. Coverage of preconception and preimplantation genetic diagnosis includes detailed laboratory protocols for biopsy procedures, polymerase chain reaction, and in situ hybridization. The book concludes with a look at the future of emerging techniques such as laser micromanipulation, gene transfer, germ-lined therapy, and nuclear transplantation.