Techniques for the Automated Optimization of High Performance Liquid Chromatography Separations

High-performance liquid chromatography (HPLC) is now a widely applied and established analytical technique used throughout industry and research. As a tool which the analyst counts as a standard method, it is appropriate that with the increasing management of laboratory methods via microcomputers, the linking of HPLC to such systems should be considered. The author does not intend to provide a fully comprehensive review of all the possible options which are available; but rather to assess the different approaches and explore the potential for further improvements. The aim is to improve the efficiency of HPLC in producing analytical results by optimising the sensitivity, selectivity and speed of separation - a selection of procedures and techniques are drawn together and a wide range of approaches are considered. "HPLC" will enable readers wishing to purchase equipment and/or software for automated optimisation to consult this single reference source with confidence for comparison of the available approaches.