Affinity chromatography, with its exquisite specificity, is based upon molecular recognition. It is a powerful tool for the purification of biomolecules. In recent years, numerous new applications and modified techniques have been derived from gro- specific interactions and biological recognition principles. An up-to-date review of the past, current, and future applications of affinity chromatography has been presented in the introductory chapter by Meir Wilchek and Irwin Chaiken. Though many of these new applications and techniques are well documented in the literature, it is often difficult to find methods that are written with the intent of helping new practitioners of affinity chromatography. This volume on Affinity Chromatography: Methods and Protocols is intended for the novice, as well as for - perts in the field. The protocols are written by experts who have developed and/or successfully employed these methods in their laboratories. Each chapter describes a specific technique, and since the book is intended to help the beginner, each technique is described simply and clearly, making sure that all relevant steps are included, assuming no previous knowledge. Each chapter contains an introduction describing the principles involved, followed by a Materials and Methods section, which lays the groundwork for the reader to conduct experiments step-by-step, in an orderly fashion. The following Notes section, which describes many of the problems that can occur, makes suggestions for overcoming them, and provides alternate procedures. These are precisely the sort of important, practical details that never seem to appear in the published literature.
Rezensionen / Stimmen
This book is a useful guide for new and experienced practitioners in the field of affinity chromatography. J. Am. Chem. Soc. The principles are first described, followed by a list of materials, and the method to be used. Probably the most useful section is that entitled Notes', which defines experimental parameters and problems that may occur. for this reason the much larger audience of experienced users may be attracted to this book if only to reduce the inordinate amount of time often spent in optimizing a specific separation. The 19 chapters of the book cover the whole range of available affinity techniques in a most concise manner. Just sufficient theory is offered to provide an understanding of the principles, and includes the most recent applications with two chapters on phage display. It is adequately indexed, concise, readable and virtually free from typographical errors. - Chromatographia the authors describe very different aspects of affinity chromatography; many parts of this book are pleasant reading. For all biochemical libraries it is simply a must. - Chembiochem The title clearly describes the scope - and the reader gets what he/she expects. the authors have compiled information in very helpful tables, which would be difficult to obtain from other sources, for instance, the binding and elution conditions for different lgG subclasses (human/mouse/rat) on Protein A. For all biochemical libraries it is simply a must. - Chembiochem this volume should be a useful handbook in any laboratory that is using, or planning to use, affinity chromatography techniques. - Biomedical Chromatography This handbook has the ambitious purpose of giving a practical overview of a field which concerns almost any researcher in biology nowadays: that of affinity chromatography. It really answers a need, as chromatographic steps are too often overlooked in the Materials and methods' sections of scientific publications. Here, protocols are precisely described with plenty of troubleshooting advice. Although the book is mostly intended for new partitioners (both industrial and academic), it will certainly also provide experts with valuable information, as the field of affinity chromatography has becomes fantastically diverse since the concept was first introduced in 1968. The focus chosen by the editors is very down-to-earth: this approach will no doubt be useful to those who seek to master technical aspects the publication of this book is an excellent and timely initiative. - Biochimie Humana's Methods in Molecular Biology series remains an excellent candidate for standing order status in any university science or medical library. This reviewer has been impressed how many new and experienced scientists like the various titles, and how many volumes have been ordered by individuals because they are so necessary for lab bench use, a four week loan is just not enough. With excellent worldwide contributors, clear and concise methods, the always-useful Notes section for each protocol, and the generally extensive and up-do-date reference lists, these books are good additions to the Humana series. Like Methods in Enzymology, Methods in Molecular Biology is indexed in PubMed, making it very easy to find the protocols within. - E-Streams Overall this text provides an excellent review of the latest trends in separating and quantifying specific molecules such as peptides and DNA and should prove to be a required text for in the age of biotechnology. - Die Pharmazie "This book fills a worthwhile niche with chapters covering major applications explicitly pertinent to cutting-edge life science research...This book stands out from the pack in that it addresses practical affinity application areas that have potentially broad use in modern technology." - Journal of medicinal Chemistry This book is an excellent up-to-date collection of carefully selected methods for affinity chromatography of small molecules, such as haptans, to supramolecular structures, such as in phage display. This book would be a most useful guide for those scientists involved in the separation and analysis of macromolecules, such as biochemists, biologists and chemists, and health scientists. - Cell Biology International It is an excellent book, and can be recommended for every laboratory involved in the synthesis and analysis of chiral compounds. Moreover, the editors have succeeded in getting internationally known leading experts to write the contributions. - Angewandte Chemie
Reihe
Sprache
Verlagsort
Zielgruppe
Für höhere Schule und Studium
Für Beruf und Forschung
Illustrationen
39 s/w Abbildungen
Illustrations
Maße
Höhe: 22.9 cm
Breite: 15.2 cm
Gewicht
ISBN-13
978-0-89603-694-9 (9780896036949)
DOI
10.1007/978-1-60327-261-2
Schweitzer Klassifikation
An Overview of Affinity Chromatography.- Weak Affinity Chromatography.- Fluidized-Bed Receptor-Affinity Chromatography.- Site-Specific Immobilization of Antibodies to Protein G-Derivatized Solid Supports.- Affinity Purification of Monoclonal Antibodies.- Protein A Mimetic (PAM) Affinity Chromatography.- Periodate Oxidation of Antibodies for Site-Selective Immobilization in Immunoaffinity Chromatography.- Mini-Antibody Affinity Chromatography of Lysozyme.- Nitrocellulose-Based Immunoaffinity Chromatography.- Immunoaffinity Chromatography.- Affinity Partitioning of Proteins.- Boronate Affinity Chromatography.- Dye-Ligand Affinity Chromatography for Protein Separation and Purification.- DNA Affinity Chromatography.- Affinity Chromatography of Pyrogens.- Western Cross Blot.- Affinity Perfusion Chromatography.- Phage Display Technology.- Phage Display Technology.