Physical Biochemistry
Principles and Applications
2nd Edition
Published on 21. January 2009
422 pages
978-0-470-03052-3 (ISBN)
Description
"As will be seen, there is not much missing here. I thought thatthe sections were well balanced, with rarely too much or too littleon a given topic...This is a text to be welcomed by both teachersand students." BIOCHEMISTRY & MOLECULAR BIOLOGY EDUCATION(on the first edition)
The second edition of this successful textbook explains thebasic principles behind the key techniques currently used in themodern biochemical laboratory and describes the pros and cons ofeach technique and compares one to another. It is non-mathematical,comprehensive and approachable for students who are not physicalchemists.
* A major update of this comprehensive, accessible introductionto physical biochemistry.
* Includes two new chapters on proteomics andbioinformatics.
* Introduces experimental approaches with a minimum ofmathematics and numerous practical examples.
* Provides a bibliography at the end of each chapter.
Written by an author with many years teaching and researchexperience, this text is a must-have for students ofbiochemistry, biophysics, molecular and life sciences and foodscience.
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Content
Preface.
Chapter 1 Introduction.
1.1 Special Chemical Requirements of Biomolecules.
1.2 Factors Affecting Analyte Structure and Stability.
1.3 Buffering Systems Used in Biochemistry.
1.4 Quantitation, Units and Data Handling.
1.5 The Worldwide Web as a Resource in PhysicalBiochemistry.
1.6 Objectives of this Volume.
References.
Chapter 2 Chromatography.
2.1 Principles of Chromatography.
2.2 Performance Parameters Used in Chromatography.
2.3 Chromatography Equipment.
2.4 Modes of Chromatography.
2.5 Open Column Chromatography.
2.6 High Performance Liquid Chromatography (HPLC).
2.7 Fast Protein Liquid Chromatography.
2.8 Perfusion Chromatography.
2.9 Membrane-Based Chromatography Systems.
2.10 Chromatography of a Sample Protein.
References.
Chapter 3 Spectroscopic Techniques.
3.1 The Nature of Light.
3.2 The Electromagnetic Spectrum.
3.3 Ultraviolet/Visible Absorption Spectroscopy.
3.4 Fluorescence Spectroscopy.
3.5 Spectroscopic Techniques Using Plane-Polarized Light.
3.6 Infrared Spectroscopy.
3.7 Nuclear Magnetic Resonance (NMR) Spectroscopy.
3.8 Electron Spin Resonance (ESR) Spectroscopy.
3.9 Lasers.
3.10 Surface Plasmon Resonance.
References.
Chapter 4 Mass Spectrometry.
4.1 Principles of Mass Spectrometry.
4.2 Mass Spectrometry of Proteins/Peptides.
4.3 Interfacing MS with other Methods.
4.4 Uses of Mass Spectrometry in Biochemistry.
References.
Chapter 5 Electrophoresis.
5.1 Principles of Electrophoresis.
5.2 Nondenaturing Electrophoresis.
5.3 Denaturing Electrophoresis.
5.4 Electrophoresis in DNA Sequencing.
5.5 Isoelectric Focusing (IEF).
5.6 Immunoelectrophoresis.
5.7 Agarose Gel Electrophoresis of Nucleic Acids.
5.8 Pulsed Field Gel Electrophoresis.
5.9 Capillary Electrophoresis.
5.10 Electroblotting Procedures.
5.11 Electroporation.
References.
Chapter 6 Three-Dimensional Structure Determination ofMacromolecules.
6.1 The Protein-Folding Problem.
6.2 Structure Determination by NMR.
6.3 Crystallization of Biomacromolecules.
6.4 X-Ray Diffraction by Crystals.
6.5 Calculation of Electron Density Maps.
6.6 Other Diffraction Methods.
6.7 Comparison of X-Ray Crystallography with Multi-DimensionalNMR.
6.8 Structural Databases.
References.
Chapter 7 Hydrodynamic Methods.
7.1 Viscosity.
7.2 Sedimentation.
7.3 Methods for Varying Buffer Conditions.
7.4 Flow Cytometry.
References.
Chapter 8 Biocalorimetry.
8.1 The Main Thermodynamic Parameters.
8.2 Isothermal Titration Calorimetry.
8.3 Differential Scanning Calorimetry.
8.4 Determination of Thermodynamic Parameters byNon-Calorimetric Means.
References.
Chapter 9 Bioinformatics.
9.1 Overview of Bioinformatics.
9.2 Sequence Databases.
9.3 Tools for Analysis of Primary Structures.
9.4 Tertiary Structure Databases.
9.5 Programs for Analysis and Visualization of TertiaryStructure Databases.
9.6 Homology Modelling.
References.
Chapter 10 Proteomics.
10.1 Electrophoresis in Proteomics.
10.2 Mass Spectrometry in Proteomics.
10.3 Chip Technologies in Proteomics.
10.4 Post-Translational Modification Proteomics.
Further Reading.
References.
Appendix 1 SI Units.
Appendix 2 The Fourier Transform.
Index.
