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This book shows the significant progress made in establishing the methodological basis for the genetic manipulation of forage and turf grasses, with particular emphasis on our most important temperate grasses, the fescues and ryegrasses. It provides detailed and beautifully illustrated descriptions of all relevant methodological aspects of molecular breeding of forage and turf grasses. The topics covered range from the establishment of plant regeneration systems from in vitro cultures, the recovery of haploids and somaclonal variants, the combination of whole or partial genomes by somatic hybridization, and the production of transgenic plants, to the development of molecular markers.
Series
Edition
Language
Place of publication
Publishing group
Illustrations
ISBN-13
978-3-642-72051-2 (9783642720512)
DOI
10.1007/978-3-642-72051-2
Schweitzer Classification
1 Introduction.- 1.1 Agronomic Importance of the Festuca-Lolium Complex..- 1.2 Distribution of Fescues and Ryegrasses.- 1.3 Biotechnology in Festuca-Lolium Improvement: General Considerations.- References.- 2 Meristem Culture.- 2.1 Introduction.- 2.2 Culture of Vegetative Meristems in Festuca and Lolium.- 2.3 Culture of Floral Meristems in Festuca and Lolium.- 2.4 Meristem Culture in Other Grasses.- 2.5 Summary and Conclusions.- References.- 3 Callus Cultures and Somaclonal Variation.- 3.1 Introduction.- 3.2 Regeneration from Callus Cultures in Festuca and Lolium.- 3.3 Somaclonal Variation in Festuca and Lolium.- 3.4 Regeneration from Callus Cultures in Other Grasses.- 3.5 Summary and Conclusions.- References.- 4 Anther Culture and Production of Haploids.- 4.1 Introduction.- 4.2 Anther Culture and Haploids in Festuca.- 4.3 Anther Culture and Haploids in Lolium.- 4.4 Anther Culture in Other Grasses.- 4.5 Summary and Conclusions.- References.- 5 Plant Regeneration from Suspension Cells and Protoplasts.- 5.1 Introduction.- 5.2 Cell Suspension and Protoplast Cultures in Festuca.- 5.3 Cell Suspension and Protoplast Cultures in Lolium.- 5.4 Suspension and Protoplast Cultures in Other Grasses.- 5.5 Summary and Conclusions.- References.- 6 Somatic Hybridization.- 6.1 Introduction.- 6.2 Somatic Hybridization in Festuca and Lolium.- 6.3 Cybridization in Festuca and Lolium.- 6.4 Somatic Hybridization in Other Grasses.- 6.5 Summary and Conclusions.- References.- 7 Transgenic Plants from Protoplasts.- 7.1 Introduction.- 7.2 Direct Gene Transfer to Protoplasts in Festuca.- 7.3 Direct Gene Transfer to Protoplasts in Lolium.- 7.4 Direct Gene Transfer to Protoplasts in Other Grasses.- 7.5 Summary and Conclusions.- References.- 8 Protoplast-Independent Production of Transgenic Plants.- 8.1 Introduction.- 8.2 Protoplast-Independent Transformation in Festuca.- 8.3 Protoplast-Independent Transformation in Lolium.- 8.4 Protoplast-Independent Transformation in Other Grasses.- 8.5 Summaryand Conclusions.- References.- 9 Molecular Markers.- 9.1 Introduction.- 9.2 Molecular Markers in Festuca and Lolium.- 9.3 Molecular Markers in Other Grasses.- 9.4 Summary and Conclusions.- References.- 10 Perspectives.- 10.1 Introduction.- 10.2 Forage Quality.- 10.3 Disease and Pest Resistance.- 10.4 Growth and Development.- 10.5 Summary and Conclusions.- References.