Evolutionary Approaches to Protein Design
Published on 14. May 2014
451 pages
978-0-08-049337-4 (ISBN)
Description
This is the first high-quality, comprehensive overview of the field of evolutionary protein design. Topics include new protein design strategies, the structures of laboratory-evolved proteins, the evolution of non-natural enzyme functions, and the theory of laboratory evolution.
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Series
Language
English
Place of publication
San Diego
United States
Product notice
Windows
Illustrations
illustrations
ISBN-13
978-0-08-049337-4 (9780080493374)
Copyright in bibliographic data and cover images is held by Nielsen Book Services Limited or by the publishers or by their respective licensors: all rights reserved.
Schweitzer Classification
Other editions
Additional editions
Frances H. Arnold
Evolutionary Approaches to Protein Design: Volume 55
Book
10/2000
Academic Press
€142.37
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Content
- Front Cover
- Evolutionary Protein Design
- Copyright Page
- CONTENTS
- PREFACE
- Chapter 1. New Functions from Old Scaffolds: How Nature Reengineers Enzymes for New Functions
- I. Introduction
- II. Defining Protein Superfamilies
- III. The Enolase Superfamily: A Paradigm for Understanding How Nature Evolved Enzymes for New Functions
- IV. Studies of Other Superfamilies and Fold Classes
- V. Conclusions
- VI. References
- Chapter 2. Evolution of Protein Functon by Domain Swapping
- I. Introduction
- II. Terminology
- III. Evolution of Proteins in Nature by Domain Swapping
- IV. Domain Swapping for Protein Engineering
- V. Methodologies
- VI. Perspective
- References
- Chapter 3. Rational Evolutionary Design: The Theory of In Vitro Protein Evolution
- I. Introduction
- II. Sequence Space and Fitness Landscapes
- III. Modeling Directed Evolution
- IV. Rational Evolutionary Design
- V. Summary and Conclusions
- References
- Chapter 4. Temperature Adaptation of Enzymes: Lessons from Laboratory Evolution
- I. Introduction
- II. Influence of Temperature on Enzymes
- III. Studies of Natural Extremophilic Enzymes
- IV. Directed Evolution
- V. Stability, Flexibility, and Catalytic Activity
- VI. Why Are Proteins Marginally Stable?
- VII. Why Are Thermophilic Enzymes Poorly Active at Low Temperature?
- VIII. Conclusions
- References
- Chapter 5. Structural Analysis of Affinity Matured Antibodies and Laboratory-Evolved Enzymes
- I. Introduction
- II. Structural Studies of Antibody Affnity Maturation
- III. Structural Studies of Enzyme Directed Evolution
- IV. Conclusions
- References
- Chapter 6. Molecular Breeding: The Natural Approach to Protein Design
- I. Introduction
- II. The Need for Better Proteins
- III. Strategies for Optimizing Proteins
- IV. Screening Is Key
- V. Beyond Proteins
- VI. Concluding Remarks
- References
- Chapter 7. Analysis of Large Libraries of Protein Mutants Using Flow Cytometry
- I. Introduction
- II. Library Screening Technologies
- III. Cell Surface Display Technologies
- IV. Library Screening by Flow Cytometry
- V. Concluding Remarks
- References
- Chapter 8. From Catalytic Asymmetric Synthesis to the Transcriptional Regulation of Genes: In Vivo and In Vitro Evolution of Proteins
- I. Introduction and Scope of Review
- II. Selecting and Evolving Therapeutic Human Antibodies
- III. Directing Evolution at the Level of Chemical Mechanism: Aldolase Antibodies and Asymmetric Catalysis
- IV. Multiple Hapten Selection: Refining the Active Site of a Catalytic Antibody by In Vitro Selection
- V. Selection and Evolution of Novel DNA-Binding Proteins: From Principles to Applications
- References
- Chapter 9. In Vitro Selection and Evolution of Proteins
- I. Introduction
- II. The Key to In Vitro Protein Evolution: Cell-Free Translation
- III. In Vitro Selection Strategies
- IV. Applications of Ribosome Display
- V. Perspectives of Directed In Vitro Evolution
- References
- AUTHOR INDEX
- SUBJECT INDEX
