Mitochondrial Function
Published on 1. January 2014
506 pages
978-0-12-801615-2 (ISBN)
Description
This new volume of Methods in Enzymology continues the legacy of this premier serial with quality chapters authored by leaders in the field. Methods to assess mitochondrial function is of great interest to neuroscientists studying chronic forms of neurodegeneration, including Parkinson's, Alzheimer's, ALS, Huntington's and other triplet repeat diseases, but also to those working on acute conditions such as stroke and traumatic brain injury. This volume covers research methods on how to assess the life cycle of mitochondria including trafficking, fusion, fission, and degradation. Multiple perspectives on the complex and difficult problem of measurement of mitochondrial reactive oxygen species production with fluorescent indicators and techniques ranging in scope from measurements on isolated mitochondria to non-invasive imaging of metabolic function.
- Continues the legacy of this premier serial with quality chapters authored by leaders in the field
- Covers research methods in biomineralization science
- Provides invaluable details on state-of-the-art methods to assess a broad array of mitochondrial functions
More details
Other editions
Additional editions
Anne Murphy | David Chan
Mitochondrial Function: Volume 547
Book
11/2014
Academic Press
€170.84
Shipment within 15-20 days
Persons
Editor
University of British Columbia University of British Columbia, Canada University of British Columbia, Canada University of British Columbia, Canada University of British Columbia, Canada University of British Columbia, Canada
National University of Singapore (NUS), Singapore Singapore Management University Singapore Management University Singapore Management University Singapore Management University Singapore Management University Singapore Management University
Content
1. High-Content Functional Genomic Screening to Identify Novel Regulators of the PINK1-Parkin PathwayAndy Cheuk-Him Ng, Stephen Baird and Robert A. Screaton2. Measurement of Mitochondrial Turnover and Life Cycle using MitoTimerKyle M. Trudeau , Roberta A.Gottlieb and Orian S. Shirihai3. Monitoring Mitophagy in Mammalian CellsYushan Zhu , Guo Chen, Linbo Chen, Du Feng, Lei Liu and Quan Chen4. Photoactivatable Green Fluorescent Protein-Based Visualization and Quantification of Mitochondrial Fusion and Mitochondrial Network Complexity in Living CellsMariusz Karbowski, Megan M. Cleland and Brian Roelofs5. Characterization of Mitochondrial Transport in NeuronsBing Zhou, Mei-Yao Lin, Tao Sun, Adam L. Knight and Zu-Hang Sheng6. Imaging of Mitochondrial Dynamics in Motor and Sensory Axons of Living Mice.Irene Bolea, Wen-Biao Gan, Giovanni Manfedi and Jordi Magranél7. Analyzing Mitochondrial Dynamics in Mouse Organotypic Slice CulturesAnh H. Pham and David C. Chan8. Analysis of Mitochondrial Traffic in DrosophilaSwathi Devireddy, Hyun Sung, Pin-Chao Liao, Elisabeth Garland-Kuntz and Peter J Hollenbeck9. In Vivo Imaging of Mitochondria in Intact Zebrafish LarvaeDominik Paquet , Gabriela Plucinska and Thomas Misgeld10. The Use of MiniSOG in the Localization of Mitochondrial Proteins Guy A. Perkins11. Assessing the Function of Mitochondria-Associated ER MembranesEstela Area-Gomez.12. Measurement of ROS Homeostasis in Isolated MitochondriaL. Tretter, and A. Ambrus13. Use of Potentiometric Fluorophores in the Measurement of Mitochondrial Reactive Oxygen SpeciesBrian M. Polster , David G. Nicholls , Shealinna X. Ge and Brian A. Roelofs14. Spatial, Temporal and Quantitative Manipulation of Intracellular Hydrogen Peroxide in Cultured CellsIshraq Alim, Renee E Haskew-Layton, Hossein Aleyasin, Hengchang Guo and Rajiv R Ratan15. Biochemical and Biophysical Methods for Studying Mitochondrial Iron MetabolismGregory P. Holmes-Hampton, Wing-Hang Tong and Tracey A. Rouault16. Analysis and Interpretation of Microplate-Based Oxygen Consumption and PH DataAjit S. Divakaruni, Alexander Paradyse1, David A. Ferrick, Anne N. Murphy ,and Martin Jastroch17. Imaging Changes in the Cytosolic ATP-to-ADP ratioMathew Tantama and Gary Yellen18. The Use of MiniSOG in the Localization of Mitochondrial Proteins Carlos T. Moraes19. Induced Pluripotent Stem Cell Derived Models for MtDNA DiseasesRiikka H Hämäläinen20. The Use of 18F-BCPP-EF as a PET Probe for Complex I Activity in the BrainHideo Tsukada21. MR OEF Imaging in MELASSheng Xie
