Cell Culture Models of Biological Barriers

General Aspects of Epithelial Cell and Tissue Culture. Basic Aspects of Cell Growth and Cell Cycle in Culture. Cell Culture Media: Selection and Standardization. Bioelectrical Characterization of Epithelial Cell (Mono)Layers and Tissues. Characterization of Transport Over Epithelial Barriers. Studying Cellular Binding and Uptake of Bioadhesive Lectins. High-throughput Epithelial Cell Culture Systems for Screening Drug Intestinal Permeability. Good Cell Culture Practice (GCCP). Regulatory Acceptance of in vitro Test Systems as an Alternative to Safety Testing in Animals. Regulatory Acceptance of in vitro Permeability Studies in the Context of the Biopharmaceutics Classification System. Models of Specific Epithelial and Endothelial Barriers Relevant to Drug Delivery. Caco-2 Cell Monolayers as a Model for Studies of Drug Transport Across Human Intestinal Epithelium. Transport Studies Using Intestinal Tissue ex vivo. Models of Alveolar Epithelium. Bronchial Epithelial Cell Cultures. In vitro Methodologies to Study Nasal Delivery Using Excised Mucosa. Cell Culture Models of the Corneal and Conjunctival Epithelium. Cell Cultures of the Retinal Pigment Epithelium to Model the Blood-retina Barrier for Retinal Drug and Gene Delivery. Human Skin and Skin Equivalents to Study Dermal Penetration and Permeation. In vitro Models of the Human Buccal Epithelium: the TR146 Cell Culture Model and the Porcine in vitro Model. Drug Transport Across the Blood-brain Barrier: A Molecular and Functional Perspective. BeWo Cells: An in vitro System Representing The Blood-placental Barrier. Emerging Tools for Studying Biological Barriers and Drug Transport. Predicting Drug Absorption by Computational Methods. Confocal and Two-photon Fluorescence Microscopy. On the Application of Scanning Force Microscopy in Cell Biology. Fluorescence Correlation Spectroscopy.