Laboratory Methods in Enzymology: Cell, Lipid and Carbohydrate

Pouring Agar Plates and Streaking or Spreading to Isolate Individual Colonies Storage of Bacteria and Yeast Cellular Fractionation - Mammalian Cells Cellular Fractionation - Yeast cells Yeast-Gene Replacement Using PCR Products Measuring RNAi knockdown using qPCR Recombineering: Using Drug Cassettes to Knock out Genes in vivo Gene Knockouts, in vivo Site-Directed Mutagenesis and Other Modifications Using the Delitto Perfetto System in Saccharomyces cerevisiae Gene targeting and Site-specific recombination in Mouse ES Cells Recombineering: Highly Efficient in vivo genetic engineering using single-strand oligos Growth Media for E. coli Saccharomyces cerevisiae Growth Media Immunohistochemistry on Freely Floating Fixed Tissue Sections Preparation of Slides for Microscopy from Frozen Tissue Sections Preparation of Formalin-fixed Paraffin-embedded Tissue for Immunohistochemistry Preparation of Cells for Microscopy using Cytospin Preparation of Cells for Microscopy using Chamber Slides and Coverslips Preparation of Cells for Microscopy using 'Cell Blocks' Enzymatic deglycosylation of glycoproteins Preparation of fatty acid or phospholipid vesicles by thin-film rehydration Vesicle extrusion through polycarbonate track-etched membranes using a hand-held mini-extruder Preparation of fatty acid micelles Reverse-phase HPLC Analysis and Purification of Small Molecules Thin Layer Chromatography ATP and GTP hydrolysis assays (TLC)