Laboratory Methods in Enzymology: Cell, Lipid and Carbohydrate: Volume 533
Academic Press
Published on 18. December 2013
Book
Hardback
408 pages
978-0-12-420067-8 (ISBN)
Description
Methods in Enzymology volumes provide an indispensable tool for the researcher. Each volume is carefully written and edited by experts to contain state-of-the-art reviews and step-by-step protocols.
In this volume, we have brought together a number of core protocols concentrating on Cell, Lipid and Carbohydrate, complementing the traditional content that is found in past, present and future Methods in Enzymology volumes.
In this volume, we have brought together a number of core protocols concentrating on Cell, Lipid and Carbohydrate, complementing the traditional content that is found in past, present and future Methods in Enzymology volumes.
More details
Series
Language
English
Place of publication
San Diego
United States
Publishing group
Elsevier Science Publishing Co Inc
Target group
Professional and scholarly
Biochemists, biophysicists, molecular biologists, analytical chemists, and physiologists
Dimensions
Height: 229 mm
Width: 152 mm
Weight
760 gr
ISBN-13
978-0-12-420067-8 (9780124200678)
Copyright in bibliographic data and cover images is held by Nielsen Book Services Limited or by the publishers or by their respective licensors: all rights reserved.
Schweitzer Classification
Other editions
Additional editions
E-Book
10/2013
Academic Press
€155.00
Available for download
Person
Content
Pouring Agar Plates and Streaking or Spreading to Isolate Individual Colonies
Storage of Bacteria and Yeast
Cellular Fractionation - Mammalian Cells
Cellular Fractionation - Yeast cells
Yeast-Gene Replacement Using PCR Products
Measuring RNAi knockdown using qPCR
Recombineering: Using Drug Cassettes to Knock out Genes in vivo
Gene Knockouts, in vivo Site-Directed Mutagenesis and Other Modifications Using the Delitto Perfetto System in Saccharomyces cerevisiae
Gene targeting and Site-specific recombination in Mouse ES Cells
Recombineering: Highly Efficient in vivo genetic engineering using single-strand oligos
Growth Media for E. coli
Saccharomyces cerevisiae Growth Media
Immunohistochemistry on Freely Floating Fixed Tissue Sections
Preparation of Slides for Microscopy from Frozen Tissue Sections
Preparation of Formalin-fixed Paraffin-embedded Tissue for Immunohistochemistry
Preparation of Cells for Microscopy using Cytospin
Preparation of Cells for Microscopy using Chamber Slides and Coverslips
Preparation of Cells for Microscopy using 'Cell Blocks'
Enzymatic deglycosylation of glycoproteins
Preparation of fatty acid or phospholipid vesicles by thin-film rehydration
Vesicle extrusion through polycarbonate track-etched membranes using a hand-held mini-extruder
Preparation of fatty acid micelles
Reverse-phase HPLC Analysis and Purification of Small Molecules
Thin Layer Chromatography
ATP and GTP hydrolysis assays (TLC)
Storage of Bacteria and Yeast
Cellular Fractionation - Mammalian Cells
Cellular Fractionation - Yeast cells
Yeast-Gene Replacement Using PCR Products
Measuring RNAi knockdown using qPCR
Recombineering: Using Drug Cassettes to Knock out Genes in vivo
Gene Knockouts, in vivo Site-Directed Mutagenesis and Other Modifications Using the Delitto Perfetto System in Saccharomyces cerevisiae
Gene targeting and Site-specific recombination in Mouse ES Cells
Recombineering: Highly Efficient in vivo genetic engineering using single-strand oligos
Growth Media for E. coli
Saccharomyces cerevisiae Growth Media
Immunohistochemistry on Freely Floating Fixed Tissue Sections
Preparation of Slides for Microscopy from Frozen Tissue Sections
Preparation of Formalin-fixed Paraffin-embedded Tissue for Immunohistochemistry
Preparation of Cells for Microscopy using Cytospin
Preparation of Cells for Microscopy using Chamber Slides and Coverslips
Preparation of Cells for Microscopy using 'Cell Blocks'
Enzymatic deglycosylation of glycoproteins
Preparation of fatty acid or phospholipid vesicles by thin-film rehydration
Vesicle extrusion through polycarbonate track-etched membranes using a hand-held mini-extruder
Preparation of fatty acid micelles
Reverse-phase HPLC Analysis and Purification of Small Molecules
Thin Layer Chromatography
ATP and GTP hydrolysis assays (TLC)