Microcharacterization of Proteins
1st Edition
Published on 11. July 2008
XVI, 268 pages
978-3-527-61570-4 (ISBN)
Description
Both a thorough introduction and laboratory guide!
This book is based on the lectures and demonstrations presented at a successful workshop organized by the authors. Expert contributions and the support of more than a dozen companies engaged in bioanalysis and instrumentation enabled the participants to familiarize themselves with the most recent developments e.g. in protein separation and characterization (including laser desorption-ionisation mass spectrometry), fragmentation and micro sequencing.
The workshop was held in the Max Planck Institute for Biochemistry at Martinsried. It helped graduate students and researchers from academia and industry both to understand and apply themselves the most sophisticated methods available. This book offers a stimulating combination of basic concepts and practical applications - and is thus useful at all levels.
This book is based on the lectures and demonstrations presented at a successful workshop organized by the authors. Expert contributions and the support of more than a dozen companies engaged in bioanalysis and instrumentation enabled the participants to familiarize themselves with the most recent developments e.g. in protein separation and characterization (including laser desorption-ionisation mass spectrometry), fragmentation and micro sequencing.
The workshop was held in the Max Planck Institute for Biochemistry at Martinsried. It helped graduate students and researchers from academia and industry both to understand and apply themselves the most sophisticated methods available. This book offers a stimulating combination of basic concepts and practical applications - and is thus useful at all levels.
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Roland Kellner | Helmut E. Meyer | Friedrich Löttspeich
Microcharacterization of Proteins
Book
11/1994
Wiley-VCH
€55.71
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Content
Contents
Section I: Overview
Microcharacterization of Proteins
Friedrich Lottspeich
Section II: Sample Preparation
Microseparation Techniques I: 2D-Gel-Electrophoresis
A. Görg and Reiner Westermeier
Microseparation Techniques II: Electroblotting
Christoph Eckerskorn
Microseparation Techniques III: Analysis of Peptides and Proteins by
Capillary Electrophoresis
Christine Schwer
Microseparation Techniques IV: High Performance Liquid Chromotography
Maria Serwe and Helmut E. Meyer
Chemical and Enzymatic Fragmentation of Proteins
Roland Kellner
Section III: Amino Acid Analysis
Amino Acid Analysis
Roland Kellner, Helmut E. Meyer and Friedrich Lottspeich
Section IV: Protein Sequence Analysis
The Edman Degradation
Friedrich Lottspeich, Tony Houthaeve and Roland Kellner
Analyzing Post translational Protein Modifications
Helmut E. Meyer
Section V: Bioanalytical Mass Spectrometry
Overview, Methods and Applications
W.D. Lehmann
Analysis of Biopolymers by Matrix-Assisted Laser Desorption/Ionization
(MALDI) Mass Spectrometry
Ute Bahr, Michael Karas and Franz Hillenkamp
Electrospray Mass Spectrometry
Jörg Metzger and Christoph Eckerskorn
Sequence Analysis of Proteins and Peptides by Mass Spectrometry
Christiane Weigt, Helmut E. Meyer and Roland Kellner
Section VI: Database Analysis
Protein Sequences and Sequence Databases
Hans-Werner Mewes and David G. George
Mass Spectrometrical Data for Protein Sequence Analysis
Matthias Mann
Computational Sequence Analysis
R. Apple and Denis F. Hochstrasser
Index
Section I: Overview
Microcharacterization of Proteins
Friedrich Lottspeich
Section II: Sample Preparation
Microseparation Techniques I: 2D-Gel-Electrophoresis
A. Görg and Reiner Westermeier
Microseparation Techniques II: Electroblotting
Christoph Eckerskorn
Microseparation Techniques III: Analysis of Peptides and Proteins by
Capillary Electrophoresis
Christine Schwer
Microseparation Techniques IV: High Performance Liquid Chromotography
Maria Serwe and Helmut E. Meyer
Chemical and Enzymatic Fragmentation of Proteins
Roland Kellner
Section III: Amino Acid Analysis
Amino Acid Analysis
Roland Kellner, Helmut E. Meyer and Friedrich Lottspeich
Section IV: Protein Sequence Analysis
The Edman Degradation
Friedrich Lottspeich, Tony Houthaeve and Roland Kellner
Analyzing Post translational Protein Modifications
Helmut E. Meyer
Section V: Bioanalytical Mass Spectrometry
Overview, Methods and Applications
W.D. Lehmann
Analysis of Biopolymers by Matrix-Assisted Laser Desorption/Ionization
(MALDI) Mass Spectrometry
Ute Bahr, Michael Karas and Franz Hillenkamp
Electrospray Mass Spectrometry
Jörg Metzger and Christoph Eckerskorn
Sequence Analysis of Proteins and Peptides by Mass Spectrometry
Christiane Weigt, Helmut E. Meyer and Roland Kellner
Section VI: Database Analysis
Protein Sequences and Sequence Databases
Hans-Werner Mewes and David G. George
Mass Spectrometrical Data for Protein Sequence Analysis
Matthias Mann
Computational Sequence Analysis
R. Apple and Denis F. Hochstrasser
Index
