Antibiotic Resistance Methods and Protocols
Published on 1. February 2008
XV, 287 pages
978-1-59259-077-3 (ISBN)
Description
At a time of rising concern about drug resistance and falling output of new antibacterial compounds, antibiotic research has once again returned to the forefront of medical science. In Antibiotic Resistance: Methods and Protocols, Stephen Gillespie and a panel of leading clinical and diagnostic microbiologists describe a series of detailed molecular and physical methods designed to study the growing problem of antibiotic resistance, as well as facilitate new antibiotic research programs for its effective redress. The techniques range widely from those that provide rapid diagnosis via DNA amplification and phage display, to those for plotting the transmission of resistant organisms and investigating their epidemiology. The methods are readily adaptable to a wide range of resistant bacterial organisms. In order to ensure successful results, each method is described in minute detail and includes tips on avoiding pitfalls.
Practical and wide-ranging, Antibiotic Resistance: Methods and Protocols provides a collection of indispensable techniques not only for illuminating the basic biology of antimicrobial resistance, but also for developing and implementing new diagnostic and epidemiological tools.
Practical and wide-ranging, Antibiotic Resistance: Methods and Protocols provides a collection of indispensable techniques not only for illuminating the basic biology of antimicrobial resistance, but also for developing and implementing new diagnostic and epidemiological tools.
Reviews / Votes
"...an excellent, well-produced book, full of protocols on a range of modern molecular techniques, applicable not only to research on antibiotics resistance, but to general microbiology....The articles are of a uniformly high standard, well illustrated and referenced, which act as logical easy to follow protocols. this book is a must for anyone involved in research on antibiotic resistance; equally all microbiologists who use modern molecular methods should consult it in the expectation of finding well-described established techniques and numerous cutting-edge developments." - MicroBiology TodayMore details
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Additional editions
Stephen H. Gillespie
Antibiotic Resistance Methods and Protocols
Book
09/2000
Humana Press Inc.
€85.59
Article exhausted; check for reprint
Content
Diagnosis of Resistance.- Multiplex Polymerase Chain Reaction Detection of vanA, vanB, vanC-1, and vanC-2/3 Genes in Enterococci.- Drug Susceptibility of Mycobacterium tuberculosisThrough the Mycolic Acid Index.- Micro-Well Phage Replication Assay for Screening Mycobacteria for Resistance to Rifampin and Streptomycin.- Application of SSCP to Identification of Resistance Mutations.- Quantitative, Single-Tube, Nested, PCR (QSTN-PCR) for Determining the Antibiotic Susceptibility of Mycobacterium tuberculosis.- Rapid Rifamycin Susceptibility Testing of Small-Volume Mycrobacterium tuberculosis Cultures by Detection of Precursor rRNA.- Detection of Penicillin Resistance in Streptococcus pneumoniae by a Seminested PCR Strategy.- Diagnosis of Penicillin Resistance by PCR-RFLP.- Detection Methods of Glycopeptide-Resistant Staphylococcus aureus I.- Detection Methods for Glycopeptide-Resistant Staphylococcus aureus II.- Multiplex PCR for the Rapid Simultaneous Speciation and Detection of Methicillin-Resistance and Genes Encoding Toxin Production in Staphylococcus aureus.- Chromogenic Detection of Aminoglycoside Phosphotransferases.- Monitoring Response to Treatment.- Quantification of M. tuberculosis DNA in Sputum During the Treatment of Pulmonary Tuberculosis.- Isolation of M. tuberculosis RNA from Sputum.- Detection of Viable Mycobacterium tuberculosis by Reverse Transcriptase-Strand Displacement Amplification of mRNA.- Molecular Epidemiology.- BOX PCR Fingerprinting for Molecular Typing of Streptococcus pneumoniae.- Restriction Fragment End Labeling Analysis: High-Resolution Genomic Typing of Streptococcus pneumoniae Isolates.- Pulse-field Gel Electrophoresis for Epidemiological Studies of Streptococcus pneumoniae.- Outer Membrane Profiles of Clonally Related Klebsiella pneumoniae.- Atomic Force Microscopy.- Biology of Resistance.- Assessing the Activity of Bacterial Multidrug Efflux Pumps.- The Use of a Continuous Culture System to Study the Antimicrobial Susceptibility of Bacteria in Biofilm.- Estimation of Mutation Rates in Antibiotic Research.- In Vitro Assessment of the Fitness of Resistant M. tuberculosis Bacteria by Competition Assay.- Purification of DNA Topoisomerases and Inhibition by Fluoroquinolones.- Site-Directed Mutagenesis to Determine Structure Function Relationships in Streptococcus pneumoniae Penicillin-Binding Protein Genes.- Detection of Low Affinity Penicillin-Binding Protein Variants in Streptococcus pneumoniae.- Transmission of Resistance.- Mobilization of Transposons.
