PCR Technology
Principles and Applications for DNA Amplification
Published on 31. December 2015
X, 246 pages
978-1-349-20235-5 (ISBN)
Description
This is an introduction to the methods and applications of polymerase chain reaction (PCR) technology, a technology developed by Erlich's group at Cetus and Cetus, and is expected to be used in all biology laboratories worldwide within the next few years.
More details
Other editions
Additional editions
Book
10/1989
Palgrave Macmillan
€29.95
Shipment within 15-20 days
Content
Part 1 Introduction to the PCR: history of PCR development; description of the PCR method - basic principles and properties of the PCR; thermostable DNA polymerases; automation; sample preparation. Part 2 Research applications - brief overview: direct sequencing using PCR; preparation of specific DNA fragments using PCR; detection of gene expression; genetic mapping using PCR amplification of individual gametes. Part 3 Diagnostic applications - brief overview: detection of pathogens; diagnosis of monogenic genetic diseases; HLA DNA typing and disease susceptibility; forensic applications; detection of activated oncogenes; future developments and applications.
