PCR Technology
Principles and Applications for DNA Amplification
Published on 1. October 1989
Book
Paperback/Softback
X, 246 pages
978-0-333-48948-2 (ISBN)
Description
This is an introduction to the methods and applications of polymerase chain reaction (PCR) technology, a technology developed by Erlich's group at Cetus and Cetus, and is expected to be used in all biology laboratories worldwide within the next few years.
More details
Edition
1989 ed.
Language
English
Place of publication
London
United Kingdom
Target group
Professional and scholarly
Product notice
Paperback (trade)
Unsewn / adhesive bound
Illustrations
X, 246 p.
Dimensions
Height: 228 mm
Width: 150 mm
Thickness: 16 mm
Weight
402 gr
ISBN-13
978-0-333-48948-2 (9780333489482)
DOI
10.1007/978-1-349-20235-5
Schweitzer Classification
Other editions
Additional editions
E-Book
12/2015
Palgrave Macmillan
€28.88
Available for download
Content
Part 1 Introduction to the PCR: history of PCR development; description of the PCR method - basic principles and properties of the PCR; thermostable DNA polymerases; automation; sample preparation. Part 2 Research applications - brief overview: direct sequencing using PCR; preparation of specific DNA fragments using PCR; detection of gene expression; genetic mapping using PCR amplification of individual gametes. Part 3 Diagnostic applications - brief overview: detection of pathogens; diagnosis of monogenic genetic diseases; HLA DNA typing and disease susceptibility; forensic applications; detection of activated oncogenes; future developments and applications.