Flow Cytometry and Cell Sorting
2nd Edition
Published on 2. November 1999
Book
Paperback/Softback
XXIII, 355 pages
978-3-540-65630-2 (ISBN)
Description
Flow cytometric analysis of molecular, biochemical, genetic and developmental parameters using cellular fluorescence techniques as well as fluorescence-activated (FACS) or magnetic (MACS) cell sorting technologies provide unique options for molecular and cellular biology. In recent years, these technologies have been considerably advanced.
In this second edition, all chapters have been updated according to the recent improvements and modifications. Further, new protocols have been added, such as on magnetic selection, magnetofluorescent liposomes, the cytometry of secreted products and microbead assays, as well as reporter gene assays for cytometry and cell selection. The recent technical developments allow diagnostic differentiation of cells according to specific gene expression, identification of rare disease-associated cells and isolation of well-defined cells at high purity for cell therapies.
In this second edition, all chapters have been updated according to the recent improvements and modifications. Further, new protocols have been added, such as on magnetic selection, magnetofluorescent liposomes, the cytometry of secreted products and microbead assays, as well as reporter gene assays for cytometry and cell selection. The recent technical developments allow diagnostic differentiation of cells according to specific gene expression, identification of rare disease-associated cells and isolation of well-defined cells at high purity for cell therapies.
More details
Series
Edition
Second Edition 2000
Language
English
Place of publication
Berlin
Germany
Publishing group
Springer Berlin
Target group
Professional and scholarly
Research
Edition type
New edition
Illustrations
XXIII, 355 p.
Dimensions
Height: 254 mm
Width: 178 mm
Thickness: 21 mm
Weight
713 gr
ISBN-13
978-3-540-65630-2 (9783540656302)
DOI
10.1007/978-3-662-04129-1
Schweitzer Classification
Other editions
Additional editions
Andreas Radbruch
Flow Cytometry and Cell Sorting
E-Book
03/2013
2nd Edition
Springer
€213.99
Available for download
Andreas Radbruch
Flow Cytometry and Cell Sorting
Book
12/2010
2nd Edition
Springer
€279.99
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Previous edition
Andreas Radbruch
Flow Cytometry and Cell Sorting
Book
10/1992
Springer
€85.59
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Content
I Flow Cytometry.- 1 Operation of a Flow Cytometer.- II Immunofluorescence.- 2 Conjugation of Fluorochromes, Haptens, and Phycobiliproteins to Antibodies.- 3 Immunofluorescence: Basic Considerations.- 4 Multicolor Immunofluorescence Analysis.- 5 Combined Intracellular and Surface Staining: Immunofluorescence of Cytokines in T Cells.- 6 Cytometry and Sorting of Live Cells According to Their Secreted Products.- 7 Cytometry of Rare Surface Molecules by Magnetofluorescent Liposomes.- 8 Scatchard Analysis by Flow Cytometry.- 9 Microbead Assay for Quantification of Neuronal Adhesion Molecule Interaction by Flow Cytometry.- III DNA and Proliferation.- 10 Preparation and Staining of Fixed Cells for High-Resolution DNA Analysis.- 11 Simultaneous Flow Cytometric Detection of Bromodeoxyuridine Incorporation and Cell Surface Marker Expression.- 12 High-Resolution Cell Cycle Analysis: The Flow Cytometric Bromodeoxyuridine-Hoechst Quenching Technique.- IV Cellular Activation and Biochemistry.- 13 Cell Activation: Indo-1 Ratiometric Analysis of Intracellular Ionized Calcium.- 14 Detection and Purification of Rare Responders by Fixed-Time Flow Cytometry.- 15 Biochemical Parameters of Cells Function.- 16 The FACS-Gal Assay.- 17 Ligand Acidification by Nonadherent Cells.- V Cell Sorting.- 18 Powerful Preselection.- 19 Magnetic Selection of Transfected Cells.- 20 High Gradient Magnetic Cells Sorting.- 21 Setup of a Flow Sorter.- 22 Sorting of Rare Cells.- 23 Fluorescence-Activated Chromosome Sorting.- 24 Analysis and Sorting of Plant Chromosomes by Flow Cytometry.- 25 Large Particle Sorting.- VI Safety.- 26 Biological and Laser Safety.- Appendices: Birgit Mechtold and Andreas Radbruch.- Suppliers.- Cytometry on the Internet.