Flow Cytometry and Cell Sorting
Published on 14. October 1992
Book
Hardback
X, 223 pages
978-3-540-55594-0 (ISBN)
Description
The practical aspects of flow cytometry and sorting are emphasized in this book which introduces the beginner to the technology and provides tips and tricks for the advanced user. The clear structure makes it easy to address specific problems fast. The chapters cover the modern applications of these procedures, with emphasis on immunofluorescence (antibody-fluorochrome conjugation, staining principles and data evaluation); the isolation of specific chromosomes, cells and fragile, large particles by magnetic and fluorescence-activated sorting; cellular biochemistry; and the dynamics of proliferation. The methods have been field-tested in recent EMBO courses on flow cytometry.
More details
Series
Language
English
Place of publication
Heidelberg
Germany
Publishing group
Springer Berlin
Target group
College/higher education
Illustrations
50 illustrations
Dimensions
Height: 216 mm
Width: 138 mm
Weight
485 gr
ISBN-13
978-3-540-55594-0 (9783540555940)
DOI
10.1007/978-3-662-02785-1
Schweitzer Classification
Other editions
New editions
Andreas Radbruch
Flow Cytometry and Cell Sorting
Book
11/1999
2nd Edition
Springer
€213.99
Shipment within 10-15 days
Additional editions
Andreas Radbruch
Flow Cytometry and Cell Sorting
E-Book
06/2013
1st Edition
Springer
€85.59
Available for download
Content
I Flow Cytometry.- 1 Operation of a Flow Cytometer.- II Immunofluorescence.- 2 Conjugation of Fluorochromes, Haptens, and Phycobiliproteins to Antibodies.- 3 Immunofluorescence: Basic Considerations.- 4 Multicolor Immunofluorescence Analysis.- 5 Combined Intracellular and Surface Staining.- 6 Scatchard Analysis by Flow Cytometry.- III DNA and Proliferation.- 7 Preparation and Staining of Cells for High-Resolution DNA Analysis.- 8 Simultaneous Flow Cytometric Detection of Bromodeoxyuridine Incorporation and Cell Surface Marker Expression.- 9 High-Resolution Cell Cycle Analysis: The Flow Cytometric Bromodeoxyuridine-Hoechst Quenching Technique.- IV Cellular Activation and Biochemistry.- 10 Cell Activation: Indo-1 Ratiometric Analysis of Intracellular Ionized Calcium.- 11 Biochemical Parameters of Cell function.- 12 Molecular Analysis of Transcriptional Control: The FACS- Galactosidase Assay.- 13 Ligand Acidification by Nonadherent Cells.- V Cell Sorting.- 14 Powerful Preselection.- 15 High Gradient Magnetic Cell Sorting.- 16 Setup of a Flow Sorter.- 17 Sorting of Rare Cells.- 18 Fluorescence-Activated Chromosome Sorting.- 19 Flow Cytometry and Sorting of Plant Chromosomes.- 20 Large Particle Sorting.- VI Safety.- 21 Biological and Laser Safety.- List of Suppliers.