Molecular Microbiology
Diagnostic Principles and Practice
3rd Edition
Will be published approx. on 1. April 2016
Book
Hardback
835 pages
978-1-55581-908-8 (ISBN)
Description
The third edition of Molecular Microbiology: Diagnostic Principles and Practice presents the latest molecular diagnostic techniques to support clinical care and basic and clinical research. The authors-all experienced researchers and diagnosticians-have conducted a comprehensive review and evaluation of this rapidly evolving field and written a book that offers a broad range of practical guidance and techniques. This text not only encapsulates the current state of the science, but also points to new avenues for research that will broaden the application and usefulness of molecular diagnostics.
Presenting the latest molecular diagnostic techniques in one comprehensive volumeThe molecular diagnostics landscape has changed dramatically since the last edition of Molecular Microbiology: Diagnostic Principles and Practice in 2011. With the spread of molecular testing and the development of new technologies and their opportunities, laboratory professionals and physicians more than ever need a resource to help them navigate this rapidly evolving field. Editors David Persing and Fred Tenover have brought together a team of experienced researchers and diagnosticians to update this third edition comprehensively, to present the latest developments in molecular diagnostics in the support of clinical care and of basic and clinical research, including next-generation sequencing and whole-genome analysis. These updates are provided in an easy-to-read format and supported by a broad range of practical advice, such as determining the appropriate type and quantity of a specimen, releasing and concentrating the targets, and eliminating inhibitors.Molecular Microbiology: Diagnostic Principles and Practice
Presents the latest basic scientific theory underlying molecular diagnostics Offers tested and proven applications of molecular diagnostics for the diagnosis of infectious diseases, including point-of-care testing Illustrates and summarizes key concepts and techniques with detailed figures and tables Discusses emerging technologies, including the use of molecular typing methods for real-time tracking of infectious outbreaks and antibiotic resistance Advises on the latest quality control and quality assurance measures Explores the increasing opportunities and capabilities of information technology
Molecular Microbiology: Diagnostic Principles and Practice is a textbook for molecular diagnostics courses that can also be used by anyone involved with diagnostic test selection and interpretation. It is also a useful reference for laboratories and as a continuing education resource for physicians.
Presenting the latest molecular diagnostic techniques in one comprehensive volumeThe molecular diagnostics landscape has changed dramatically since the last edition of Molecular Microbiology: Diagnostic Principles and Practice in 2011. With the spread of molecular testing and the development of new technologies and their opportunities, laboratory professionals and physicians more than ever need a resource to help them navigate this rapidly evolving field. Editors David Persing and Fred Tenover have brought together a team of experienced researchers and diagnosticians to update this third edition comprehensively, to present the latest developments in molecular diagnostics in the support of clinical care and of basic and clinical research, including next-generation sequencing and whole-genome analysis. These updates are provided in an easy-to-read format and supported by a broad range of practical advice, such as determining the appropriate type and quantity of a specimen, releasing and concentrating the targets, and eliminating inhibitors.Molecular Microbiology: Diagnostic Principles and Practice
Presents the latest basic scientific theory underlying molecular diagnostics Offers tested and proven applications of molecular diagnostics for the diagnosis of infectious diseases, including point-of-care testing Illustrates and summarizes key concepts and techniques with detailed figures and tables Discusses emerging technologies, including the use of molecular typing methods for real-time tracking of infectious outbreaks and antibiotic resistance Advises on the latest quality control and quality assurance measures Explores the increasing opportunities and capabilities of information technology
Molecular Microbiology: Diagnostic Principles and Practice is a textbook for molecular diagnostics courses that can also be used by anyone involved with diagnostic test selection and interpretation. It is also a useful reference for laboratories and as a continuing education resource for physicians.
Reviews / Votes
"Molecular methods have become indispensable for the rapid and accurate diagnosis of infections, and this book provides an authoritative guide to the field. I highly recommend it." Ferric C. Fang, MD, Professor of Laboratory Medicine and Microbiology, Adjunct Professor of Medicine (Infectious Diseases), University of Washington School of Medicine & Director, Harborview Medical Center Clinical Microbiology LaboratoryMore details
Series
Language
English
Place of publication
Washington DC
United States
Target group
College/higher education
Professional and scholarly
graduate students, laboratory personnel, physicians (continuing education resource)
Edition type
New edition
Dimensions
Height: 284 mm
Width: 215 mm
Thickness: 38 mm
Weight
1909 gr
ISBN-13
978-1-55581-908-8 (9781555819088)
Schweitzer Classification
Other editions
Additional editions
David H. Persing | Fred C. Tenover | Randall T. Hayden
Molecular Microbiology
Diagnostic Principles and Practice
E-Book
07/2020
3rd Edition
Wiley
€171.99
Available for download
David H. Persing | Fred C. Tenover | Randall T. Hayden
Molecular Microbiology
Diagnostic Principles and Practice
E-Book
04/2016
3rd Edition
Wiley
€171.99
Available for download
Person
Dr. Persing is the Executive Vice President and Chief Medical and Technology Officer at Cepheid and Consulting Professor of Pathology at Stanford University School of Medicine. Over the past 25 years, he has held leadership roles in academia and industry, starting with the design, implementation, and operation of the first PCR laboratory at the Mayo Clinic in the early 1990s. His interest in the democratization of molecular diagnostic methods has been longstanding, starting in 1993 with publication the first widely adopted textbook to include PCR protocols and guidelines for laboratory operations. His more recent work has focused on the enablement of molecular diagnostic technology to meet global needs. He has published over 300 peer reviewed articles and reviews, many of which are considered seminal works in the field. He has served as Editor in Chief for 5 textbooks published by ASM Press. Fred C. Tenover is Vice President of Scientific Affairs, Cepheid; Consulting Professor, Pathology, Stanford University School of Medicine; and Adjunct Professor, Epidemiology, Emory University. Prior to joining Cepheid in 2008, Dr. Tenover served as Associate Director, Laboratory Science, Healthcare Quality Promotion Division and Director, Office of Antimicrobial Resistance, Centers for Disease Control and Prevention. He is a Diplomate of the American Board of Medical Microbiology and a Fellow of both the American Academy of Microbiology and the Infectious Disease Society of America. He has authored over 350 peer-reviewed journal articles and book chapters and has edited 10 books.
Content
ContributorsPreface
Section I: Novel and Emerging Technologies1 Nucleic Acid Amplification Methods Overview 2 Application of Identification of Bacteria by DNA Target Sequencing in a Clinical Microbiology Laboratory 3 Microbial Whole-Genome Sequencing: Applications in Clinical Microbiology and Public Health 4 Digital PCR and Its Potential Application to Microbiology 5 Massively Parallel DNA Sequencing and Microbiology 6 Next-Generation Sequencing 7 Pathogen Discovery 8 Matrix-Assisted Laser Desorption Ionization-Time of Flight Mass Spectrometry for Microbial Identification in Clinical Microbiology 9 Multiplex Technologies
Section II: Metagenomics: Implications for Diagnostics10 The Skin Microbiome: Insights into Potential Impact on Diagnostic Practice 11 The Gastrointestinal Microbiome 12 The Vaginal Microbiome 13 Microbial Communities of the Male Urethra 14 The Human Virome in Health and Disease
Section III: Health Care-Associated Infections15 Molecular Detection of Staphylococcus aureus Colonization and Infection 16 Molecular Diagnostics for Clostridium difficile 17 Overview of Molecular Diagnostics in Multiple-Drug-Resistant Organism Prevention: Focus on Multiple-Drug-Resistant Gram-Negative Bacterial Organisms 18 Detection of Vancomycin-Resistant Enterococci
Section IV: Molecular Diagnostics and Public Health19 The Impact of Molecular Diagnostics on Surveillance of Foodborne Infections 20 Role of Molecular Methods in Improving Public Health Surveillance of Infections Caused by Antimicrobial-Resistant Bacteria in Health Care and Community Settings 21 Molecular Diagnostics: Huge Impact on the Improvement of Public Health in China 22 Surveillance and Epidemiology of Norovirus Infections 23 Molecular Diagnostic Assays for the Detection and Control of Zoonotic Diseases
Section V: Syndromic Diagnostics24 Molecular Approaches to the Diagnosis of Meningitis and Encephalitis 25 Using Nucleic Acid Amplification Techniques in a Syndrome-Oriented Approach: Detection of Respiratory Agents 26 Molecular and Mass Spectrometry Detection and Identification of Causative Agents of Bloodstream Infections 27 Molecular Diagnosis of Gastrointestinal Infections 28 Diagnostic Approaches to Genitourinary Tract Infections 29 Syndromic Diagnostic Approaches to Bone and Joint Infections
Section VI: Virology30 Molecular Detection and Characterization of Human Immunodeficiency Virus Type 1 31 Molecular Detection and Characterization of Hepatitis C Virus 32 Molecular Detection and Characterization of Hepatitis B Virus 33 Molecular Detection of Human Papillomaviruses 34 Molecular Diagnostics for Viral Infections in Transplant Recipients
Section VII: Fungi and Protozoa35 Molecular Detection and Identification of Fungal Pathogens 36 Molecular Approaches for Diagnosis of Chagas' Disease and Genotyping of Trypanosoma cruzi 37 Molecular Approaches for Diagnosis of Malaria and the Characterization of Genetic Markers for Drug Resistance 38 Molecular Detection of Gastrointestinal Parasites
Section VIII: Point-of-Care/Near-Care Diagnostics39 Molecular Diagnostics and the Changing Face of Point-of-Care 40 Point-of-Care Technologies for the Diagnosis of Active Tuberculosis 41 Molecular Diagnostics for Use in HIV/AIDS Care and Treatment in Resource-Limited Settings 42 Rapid Point-of-Care Diagnosis of Malaria and Dengue Infection
Section IX: The Host and Host Response43 Implications of Pharmacogenetics for Antimicrobial Prescribing 44 Exploiting MicroRNA (miRNA) Profiles for Diagnostics 45 Host Response in Human Immunodeficiency Virus Infection 46 Biomarkers of Gastrointestinal Host Responses to Microbial Infections
Section X: Information Technology47 Point-of-Care Medical Device Connectivity: Developing World Landscape 48 WHONET: Software for Surveillance of Infecting Microbes and Their Resistance to Antimicrobial Agents 49 Cloud-Based Surveillance, Connectivity, and Distribution of the GeneXpert Analyzers for Diagnosis of Tuberculosis (TB) and Multiple-Drug-Resistant TB in South Africa
Section XI: Quality Assurance50 Molecular Method Verification 51 Molecular Microbiology Test Quality Assurance and Monitoring 52 Proficiency Testing and External Quality Assessment for Molecular Microbiology 53 Practices of Sequencing Quality Assurance 54 Verification and Validation of Matrix-Assisted Laser Desorption Ionization Time of Flight Mass Spectrometry-Based Protocols
Section XII: The Business of Diagnostics55 Improved Diagnostics in Microbiology: Developing a Business Case for Hospital Administration 56 Molecular Diagnostics and the Changing Legal Landscape
Index
Section I: Novel and Emerging Technologies1 Nucleic Acid Amplification Methods Overview 2 Application of Identification of Bacteria by DNA Target Sequencing in a Clinical Microbiology Laboratory 3 Microbial Whole-Genome Sequencing: Applications in Clinical Microbiology and Public Health 4 Digital PCR and Its Potential Application to Microbiology 5 Massively Parallel DNA Sequencing and Microbiology 6 Next-Generation Sequencing 7 Pathogen Discovery 8 Matrix-Assisted Laser Desorption Ionization-Time of Flight Mass Spectrometry for Microbial Identification in Clinical Microbiology 9 Multiplex Technologies
Section II: Metagenomics: Implications for Diagnostics10 The Skin Microbiome: Insights into Potential Impact on Diagnostic Practice 11 The Gastrointestinal Microbiome 12 The Vaginal Microbiome 13 Microbial Communities of the Male Urethra 14 The Human Virome in Health and Disease
Section III: Health Care-Associated Infections15 Molecular Detection of Staphylococcus aureus Colonization and Infection 16 Molecular Diagnostics for Clostridium difficile 17 Overview of Molecular Diagnostics in Multiple-Drug-Resistant Organism Prevention: Focus on Multiple-Drug-Resistant Gram-Negative Bacterial Organisms 18 Detection of Vancomycin-Resistant Enterococci
Section IV: Molecular Diagnostics and Public Health19 The Impact of Molecular Diagnostics on Surveillance of Foodborne Infections 20 Role of Molecular Methods in Improving Public Health Surveillance of Infections Caused by Antimicrobial-Resistant Bacteria in Health Care and Community Settings 21 Molecular Diagnostics: Huge Impact on the Improvement of Public Health in China 22 Surveillance and Epidemiology of Norovirus Infections 23 Molecular Diagnostic Assays for the Detection and Control of Zoonotic Diseases
Section V: Syndromic Diagnostics24 Molecular Approaches to the Diagnosis of Meningitis and Encephalitis 25 Using Nucleic Acid Amplification Techniques in a Syndrome-Oriented Approach: Detection of Respiratory Agents 26 Molecular and Mass Spectrometry Detection and Identification of Causative Agents of Bloodstream Infections 27 Molecular Diagnosis of Gastrointestinal Infections 28 Diagnostic Approaches to Genitourinary Tract Infections 29 Syndromic Diagnostic Approaches to Bone and Joint Infections
Section VI: Virology30 Molecular Detection and Characterization of Human Immunodeficiency Virus Type 1 31 Molecular Detection and Characterization of Hepatitis C Virus 32 Molecular Detection and Characterization of Hepatitis B Virus 33 Molecular Detection of Human Papillomaviruses 34 Molecular Diagnostics for Viral Infections in Transplant Recipients
Section VII: Fungi and Protozoa35 Molecular Detection and Identification of Fungal Pathogens 36 Molecular Approaches for Diagnosis of Chagas' Disease and Genotyping of Trypanosoma cruzi 37 Molecular Approaches for Diagnosis of Malaria and the Characterization of Genetic Markers for Drug Resistance 38 Molecular Detection of Gastrointestinal Parasites
Section VIII: Point-of-Care/Near-Care Diagnostics39 Molecular Diagnostics and the Changing Face of Point-of-Care 40 Point-of-Care Technologies for the Diagnosis of Active Tuberculosis 41 Molecular Diagnostics for Use in HIV/AIDS Care and Treatment in Resource-Limited Settings 42 Rapid Point-of-Care Diagnosis of Malaria and Dengue Infection
Section IX: The Host and Host Response43 Implications of Pharmacogenetics for Antimicrobial Prescribing 44 Exploiting MicroRNA (miRNA) Profiles for Diagnostics 45 Host Response in Human Immunodeficiency Virus Infection 46 Biomarkers of Gastrointestinal Host Responses to Microbial Infections
Section X: Information Technology47 Point-of-Care Medical Device Connectivity: Developing World Landscape 48 WHONET: Software for Surveillance of Infecting Microbes and Their Resistance to Antimicrobial Agents 49 Cloud-Based Surveillance, Connectivity, and Distribution of the GeneXpert Analyzers for Diagnosis of Tuberculosis (TB) and Multiple-Drug-Resistant TB in South Africa
Section XI: Quality Assurance50 Molecular Method Verification 51 Molecular Microbiology Test Quality Assurance and Monitoring 52 Proficiency Testing and External Quality Assessment for Molecular Microbiology 53 Practices of Sequencing Quality Assurance 54 Verification and Validation of Matrix-Assisted Laser Desorption Ionization Time of Flight Mass Spectrometry-Based Protocols
Section XII: The Business of Diagnostics55 Improved Diagnostics in Microbiology: Developing a Business Case for Hospital Administration 56 Molecular Diagnostics and the Changing Legal Landscape
Index