
Protein Purification
Principles, High Resolution Methods, and Applications
Wiley (Publisher)
2nd Edition
Published on 1. June 1997
Book
Hardback
XIV, 698 pages
978-0-471-18626-7 (ISBN)
Article exhausted; check for reprint
Description
Praise for the First Edition of Protein Purification:
"Contains much useful information for both the novice in protein purification and the experienced practitioner. [an] excellent practical manual." -Trends in Analytical Chemistry.
"[This book is] easy to read and gives a comprehensive description of all major methods of protein purification. a much welcomed source of information for protein biochemists." -FEBS Letters.
This thoroughly revised and updated edition of Protein Purification provides state-of-the-art coverage of chromatographic and electrophoretic protein separation and characterization methods. Balancing theory, procedures, and applications, it offers professionals and students in biochemistry, organic chemistry, and analytical chemistry quick access to a wide range of important techniques, including:
* Gel filtration and ion exchange chromatography
* Affinity and covalent chromatography
* Reversed phase and hydrophobic interaction chromatography
* Electrophoresis in gels
* Immunoelectrophoresis and capillary electrophoresis
* Protein mapping by two-dimensional polyacrylamide gel electrophoresis
* Protein elution and blotting methods
* Large-scale electrophoresis processes.
"Contains much useful information for both the novice in protein purification and the experienced practitioner. [an] excellent practical manual." -Trends in Analytical Chemistry.
"[This book is] easy to read and gives a comprehensive description of all major methods of protein purification. a much welcomed source of information for protein biochemists." -FEBS Letters.
This thoroughly revised and updated edition of Protein Purification provides state-of-the-art coverage of chromatographic and electrophoretic protein separation and characterization methods. Balancing theory, procedures, and applications, it offers professionals and students in biochemistry, organic chemistry, and analytical chemistry quick access to a wide range of important techniques, including:
* Gel filtration and ion exchange chromatography
* Affinity and covalent chromatography
* Reversed phase and hydrophobic interaction chromatography
* Electrophoresis in gels
* Immunoelectrophoresis and capillary electrophoresis
* Protein mapping by two-dimensional polyacrylamide gel electrophoresis
* Protein elution and blotting methods
* Large-scale electrophoresis processes.
More details
Edition
2., Auflage
Language
English
Place of publication
New York
United States
Publishing group
John Wiley and Sons Ltd
Target group
College/higher education
Professional and scholarly
Biochemiker, Biotechnologen, Molekularbiologen, Medizinische Chemiker, Bakteriologen
Edition type
Revised edition
Illustrations
170
50 s/w Tabellen, 170 s/w Abbildungen
figures
Dimensions
Height: 24.2 cm
Width: 16.2 cm
Thickness: 38 mm
Weight
1142 gr
ISBN-13
978-0-471-18626-7 (9780471186267)
Schweitzer Classification
Other editions
New editions

Book
04/2011
3rd Edition
Wiley
€158.50
Article not available at the moment
Previous edition
Jan Ch Janson | Lars Ryden
Protein Purification
Principles, High Resolution Methods, and Applications
Book
12/1989
Wiley-VCH
€63.14
Article exhausted; check for reprint
Persons
JAN-CHRISTER JANSON is Adjunct Professor of Technical Biochemistry at Uppsala University and Principal Scientist at Amersham Pharmacia Biotech in Sweden.
LARS RYDEN is Assistant Professor of Biochemistry at Uppsala University in Sweden.
LARS RYDEN is Assistant Professor of Biochemistry at Uppsala University in Sweden.
Content
Introduction to Protein Purification (B. Ersson, et al.).
CHROMATOGRAPHY.
Introduction to Chromatography (J.-C.
Janson & J.-Å.
Jönsson).
Gel Filtration (L. Hagel).
Ion Exchange Chromatography (E. Karlsson, et al.).
Chromatofocusing (T. Hutchens).
High-Resolution Reversed-Phase Chromatography (M. Hearn).
Hydrophobic Interaction Chromatography (K.-O.
Eriksson).
Immobilized Metal Ion Affinity Chromatography (L. Kågedal).
Covalent Chromatography (J. Carlsson, et al.).
Affinity Chromatography (J. Carlsson, et al.).
Affinity Partitioning of Proteins Using Aqueous Two-Phase Systems (G. Johansson).
ELECTROPHORESIS.
Electrophoresis in Gels (T. Låås).
Isoelectric Focusing (T. Låås).
Immunoelectrophoresis (J. Lizana).
Protein Mapping by Two-Dimensional Polyacrylamide Electrophoresis (R. Westermeier & A. Görg).
Protein Elution and Blotting Techniques (R. Westermeier).
Capillary Electrophoretic Separations (W. Thormann).
Large-Scale Electrophoretic Processes (W. Thormann).
Index.
CHROMATOGRAPHY.
Introduction to Chromatography (J.-C.
Janson & J.-Å.
Jönsson).
Gel Filtration (L. Hagel).
Ion Exchange Chromatography (E. Karlsson, et al.).
Chromatofocusing (T. Hutchens).
High-Resolution Reversed-Phase Chromatography (M. Hearn).
Hydrophobic Interaction Chromatography (K.-O.
Eriksson).
Immobilized Metal Ion Affinity Chromatography (L. Kågedal).
Covalent Chromatography (J. Carlsson, et al.).
Affinity Chromatography (J. Carlsson, et al.).
Affinity Partitioning of Proteins Using Aqueous Two-Phase Systems (G. Johansson).
ELECTROPHORESIS.
Electrophoresis in Gels (T. Låås).
Isoelectric Focusing (T. Låås).
Immunoelectrophoresis (J. Lizana).
Protein Mapping by Two-Dimensional Polyacrylamide Electrophoresis (R. Westermeier & A. Görg).
Protein Elution and Blotting Techniques (R. Westermeier).
Capillary Electrophoretic Separations (W. Thormann).
Large-Scale Electrophoretic Processes (W. Thormann).
Index.