Receptor Research Methods
1st Edition
Published on 1. August 1995
Book
Paperback/Softback
500 pages
978-3-7186-5671-4 (ISBN)
Description
This text describes and evaluates the techniques used in receptor research. The range and scope of receptor studies becomes apparent through giving examples of the use of these techniques in overcoming problems of receptor identification in the brain and other tissues. Measurement of receptor action is done directly and also indirectly, through cellular expression of receptor activation by appropriate ligands. The role of the receptor in physiological and pathological states is also discussed in the book. The emphasis is on how receptors can be used to tackle a problem, rather than studying the receptor per se.
More details
Language
English
Place of publication
London
United Kingdom
Target group
College/higher education
Professional and scholarly
Illustrations
Illustrations
Weight
816 gr
ISBN-13
978-3-7186-5671-4 (9783718656714)
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Schweitzer Classification
Other editions
Additional editions
Greenstein
Receptor Research Methods
Book
08/1995
1st Edition
Harwood-Academic Publishers
€146.08
Article exhausted; check different version
Content
Section 1: Extracellular Receptors: Binding Techniques for Catecholamine Receptors; alpha 2 Adrenoreceptor Signal Transduction at Sympathetic Nerve Endings; Adenosine Receptors on Human Airway Epithelium; Histamine and Adenosine Receptor-mediated inositol Phosphate Accumulation and calcium mobilisation in DDT1 MF-2 cellmonolayers; Cloning and analysis of activin receptors; Characterization of angiotensin receptors; Proliferation of a murine IL-2 dependent cell line to measure altered IL-2 receptor function induced by tunicamycin and sialidase; The use of fluorescent lipid analogues to study endocytosis of glycosphingolipids; Cell culture techniques. Section II: Intracellular Receptors: Kinetic analysis of estrogen receptor interaction in intact cells; A dual parameter flow cytometry assay for progesterone receptors and DNA indices;. Measurement of steroid hormone receptors in rat and mouse brain and other tissues; Cellular modulators of steroid receptor phosphorylation and function; Design of estrogen agonists and antagonists; Gene expression as a target for drug discovery: Identification of endogenous ligands and novel drug candidates for intracellular receptors.