RNA Methodologies
A Laboratory Guide for Isolation and Characterization
2nd Edition
Published on 24. September 1998
Book
Paperback/Softback
533 pages
978-0-12-249695-0 (ISBN)
Description
This laboratory guide is a collection of tested and optimized laboratory protocols for the isolation and characterization of eukaryotic RNA. This edition includes a variety of RNA-based PCR techniques, such as differential display PCR, and transcript quantification by competitive (mimic) PCR. The book focuses on how these methodologies can be used in the study of transcriptional and posttranscriptional regulation of eukaryotic genes. The liberal incorporation of flow charts, tables, and graphs facilitate learning and assist in the planning phases of projects. It is a valuable resource for investigators who wish to characterize cellular biochemistry.
Reviews / Votes
Each of the protocols is written relatively independent of the rest of the text, allowing the reader to immediately begin using a procedure without having to study the text from cover to cover... Very few other molecular biology guide books are available that cover the isolation and manipulation of RNA as exhaustingly as does this one. This book would be highly valuable in any research facility engaged in molecular biology. --Mark A. Jandreski, Loyola University Medical Center, DOODY'S ELECTRONIC JOURNALMore details
Edition
2nd Revised edition
Language
English
Place of publication
San Diego
United States
Publishing group
Elsevier Science Publishing Co Inc
Target group
Professional and scholarly
Edition type
Revised edition
Illustrations
b&w illustrations, charts, graphs, tables
Dimensions
Height: 229 mm
Width: 152 mm
Weight
744 gr
ISBN-13
978-0-12-249695-0 (9780122496950)
Copyright in bibliographic data is held by Nielsen Book Services Limited or its licensors: all rights reserved.
Schweitzer Classification
Other editions
New editions
Book
05/2005
3rd Edition
Academic Press
€95.32
Article exhausted; check for reprint
Previous edition
Book
01/1993
Academic Press
€49.46
Article exhausted; check for reprint
Content
Preface. RNA and the Cellular Biochemistry Revisited. Transcription and the Organization of Eukaryotic Gene Sequences. Messenger RNA. Resilient Ribonucleases. RNA Isolation Strategies. Isolation of Polyadenylated RNA. Quality Control for RNA Preparations. Dot Blot Analysis. Electrophoresis of RNA. Photodocumentation and Image Analysis. The Northern Analysis. Nucleic Acid Probe Technology. Practical Nucleic Acid Hybridization. Principles of Detection. RT PCR. Messenger RNA Differential Display. Quantitative PCR Techniques. Quantification of Specific mRNAs by Nuclease Protection. Analysis of Nuclear RNA. An RNA Paradigm. Epilogue. A Few Pearls of Wisdom. Appendices: Phenol Preparation. Disposal of Ethidium Bromide and SYBR Green Solutions. DNase I Removal of DNA from an RNA Sample. RNase Incubation to Remove RNA from a DNA sample. Deionization of Formamide, Formaldehyde, and Glyoxal. Silanizing Centrifuge Tubes and Glassware. Centrifugation as a Mainstream Tool for the Molecular Biologist. Trypsinization Protocol for Anchorage-Dependent Cells. Isolation of High-Molecular-Weight DNA by the Salting-Out Procedure. RNA Isolation from Plant Tissue. Electrophoresis: Principles, Parameters, and Safety. Polyacrylamide Gels. Internal Controls. Trade Citations. Selected Suppliers of Equipment, Reagents, and Services. Glossary. Index.