Pichia Protocols

Vectors and Strains for Expression.- DNA-Mediated Transformation.- Rational Design and Optimization of Fed-Batch and Continuous Fermentations.- Saturation of the Secretory Pathway by Overexpression of a Hookworm (Necator americanus) Protein (Na-ASP1).- Purification of the N- and C-Terminal Subdomains of Recombinant Heavy Chain Fragment C of Botulinum Neurotoxin Serotype C.- Rapid Screening of Chromatography Resins for the Purification of Proteins.- Characterization of O-Linked Saccharides on Glycoproteins.- Modification of the N-Glycosylation Pathway to Produce Homogeneous, Human-Like Glycans Using GlycoSwitch Plasmids.- N-Linked Glycan Characterization of Heterologous Proteins.- Heavy Labeling of Recombinant Proteins.- Selenomethionine Labeling of Recombinant Proteins.- Selective Isotopic Labeling of Recombinant Proteins Using Amino Acid Auxotroph Strains.- Classical Genetics.- Identification of Pexophagy Genes by Restriction Enzyme-Mediated Integration.- Characterization of Protein-Protein Interactions.- Localization of Proteins and Organelles Using Fluorescence Microscopy.- Fluorescence Microscopy and Thin-Section Electron Microscopy.