Pichia Protocols
Published on 1. January 1998
Book
Hardback
284 pages
978-0-89603-421-1 (ISBN)
Description
David Higgins and James Cregg compile for the first time an all-inclusive collection of key experimental procedures to manipulate Pichia pastoris for gene expression purposes. The methods - developed by world-class researchers in their own laboratories - contain detailed descriptions of available expression strains, as well as vectors and step-by-step instructions for the construction of expression strains and for their use in the production of foreign proteins from shake-flask or fermentor cultures. General procedures for the purification of foreign proteins are also provided, along with protocols for analyzing foreign protein products for glycosylation and subcellular location. Its detailed instructions will permit any researcher in molecular biology to quickly construct an expression strain that will produce a desired foreign protein, and to use this state-of-the-art molecular and cellular biological system successfully in every area of biological research today.
Reviews / Votes
In this valuable Methods in Molecular Biology volume, the editors have assembled an international cast of Pichia experts who provide detailed protocols, advice, and theory on recombinant expression in this methylotrophic yeast...a focused writing style that is enjoyable to read. Molecular geneticists are afforded the essential information to achieve success in vector construction, transformation and screening. - The Quarterly Review of BiologyMore details
Series
Language
English
Place of publication
Totowa, NJ
United States
Target group
College/higher education
Professional and scholarly
Dimensions
Height: 152 mm
Width: 229 mm
Weight
713 gr
ISBN-13
978-0-89603-421-1 (9780896034211)
Copyright in bibliographic data is held by Nielsen Book Services Limited or its licensors: all rights reserved.
Schweitzer Classification
Other editions
New editions
James M. Cregg
Pichia Protocols
Book
08/2007
2nd Edition
Humana Press Inc.
€106.99
Shipment within 15-20 days
Content
Introduction to Pichia pastoris David R. Higgins and James M. Cregg. Classical Genetic Manipulation James M. Cregg Shigang Shen Monique Johnson and Hans R. Waterham. Transformation James M. Cregg and Kimberly A. Russell. Small Vectors for Expression Based on Dominant Drug Resistance with Direct Multicopy Selection David R. Higgins Katie Busser John Comiskey Peter S. Whittier Thomas J. Purcell and James P. Hoeffler. The Generation of Multicopy Recombinant Strains Mike Romanos Carol Scorer Koti Sreekrishna and Jeff Clare. Isolation of Nucleic Acids David J. Miles Katie Busser Christine Stalder and David R. Higgins. Generation of Protease- Deficient Strains and Their Use in Heterologous Protein Expression Martin A. G. Gleeson Christopher E. White David P. Meininger and Elizabeth A. Komives. Glycosylation Profiling of Heterologous Proteins Jos' Cremata Raquel Montesino Omar Quintero and Rossana Garc???a.High Cell-Density Fermentation Jayne Stratton Vijay Chiruvolu and Michael Meagher. Use of Pichia pastoris as a Model Eukaryotic System: Peroxisome Biogenesis Klaas Nico Faber Ype Elgersma John A. Heyman Antonius Koller Georg H. L?ers William M. Nuttley Stanley R. Terlecky Thibaut J. Wenzel and Suresh Subramani. Secretion of Recombinant Human Insulin-Like Growth Factor I (IGF-I) Russell A. Brierley. Secretion of scFv Antibody Fragments Hermann Gram Rita Schmitz and R?diger Ridder. Expression of Tetanus Toxin Fragment C Jeff Clare Koti Sreekrishna and Mike Romanos. Expression of EGF and HIV Envelope Glycoprotein Jeff Clare Carol Scorer Rich Buckholz and Mike Romanos. Expression of an Integral Membrane Protein the 5HT5A Receptor H. Markus Weia Winfried Haase and Helmut Reil"nder. Localization of the myc-Tagged 5HT5A Receptor by Immunogold Staining of Ultrathin Sections Winfried Haase H. Markus Weia and Helmut Reil"nder. Appendix: Foreign Proteins Expressed in P. pastoris.Index.