Molecular Diagnostics

Part I. Basic Molecular Biology. A Historical Perspective on the Clinical Diagnostic Laboratory, Robert E. Moore. An Overview of Nucleic Acid Chemistry, Structure, and Function: The Foundations of Molecular Biology, William B. Coleman. Basic Nucleic Acid Procedures, R. Keith Esch. Part II. Molecular Technologies. Nucleic Acid Blotting Techniques: Theory and Practice, Sharon Collins Presnell. DNA Amplification Techniques: An Overview, David H. Sorscher. RT-PCR: Quantitative and Diagnostic PCR in the Analysis of Gene Expression, Robert B. Sisk. PCR-Based Methods for Mutation Detection, Elizabeth M. Rohlfs and W. Edward Highsmith, Jr. Nucleic Acid Hybridization and Amplification In Situ: Principles and Applications in Molecular Pathology, Matthew S. Cowlen. Gene Therapy, Jeffrey S. Bartlett. Part III. Applications to Molecular Pathology. An Overview of Molecular Genetics, Gregory J. Tsongalis. Genetic Basis of Neurologic and Neuromuscular Diseases, Myra J. Wick, Pamela A. Crifasi, Zhenyuan Wang, and Stephen N. Thibodeau. Molecular Mechanisms of Endocrine Disorders, Bruce F. Bower and Carl D. Malchoff. Molecular Pathogenesis of Cardiovascular Disease, John H. Contois and Juch Chin Huang. Molecular Mutations in Human Neoplastic Disease: Application of Nucleic Acid Technology in the Assessment of Familial Risk, Diagnosis, and Prognosis of Human Cancers, William B. Coleman and Gregory J. Tsongalis. Molecular Genetics and the Diagnosis of Hematological Malignancies, William N. Rezuke and Evelyn C. Abernathy. Molecular Techniques in Laboratory Diagnosis of Infectious Diseases, Jaber Aslanzadeh. Part IV. Issues for the Clinical Molecular Pathology Laboratory. Quality Control Issues for the Clinical Molecular Pathologist, Daniel H. Farkas. Prospects for the Future Role of the Clinical Laboratory, Lawrence M. Silverman. Index.