
Protein Purification
Philip Bonner(Author)
CRC Press
2nd Edition
Published on 16. August 2018
Book
Paperback/Softback
269 pages
978-0-8153-4488-9 (ISBN)
Description
This second edition of Protein Purification provides a guide to the major chromatographic techniques, including non-affinity absorption techniques, affinity procedures, non-absorption techniques and methods for monitoring protein purity. The new edition of the book has been organized to encourage incremental learning about the topic, starting with the properties of water, progressing through the characteristics of amino acids and proteins which relate to the purification process. There is an overview of protein strategy and equipment, followed by discussions and examples of each technique and their applications.
The basic theory and simple explanations given in Protein Purification make it an ideal handbook for final year undergraduates, and postgraduates, who are conducting research projects. It will also be a useful guide to more experienced researchers who need a good overview of the techniques and products used in protein purification.
Key Features
* Guide to the major techniques used in protein purification
* Includes flowcharts to help the reader slect the best purification strategy
* Contains step-by-step protocols that guide the reader through each technique and its use
* Includes exercises and solutions
The basic theory and simple explanations given in Protein Purification make it an ideal handbook for final year undergraduates, and postgraduates, who are conducting research projects. It will also be a useful guide to more experienced researchers who need a good overview of the techniques and products used in protein purification.
Key Features
* Guide to the major techniques used in protein purification
* Includes flowcharts to help the reader slect the best purification strategy
* Contains step-by-step protocols that guide the reader through each technique and its use
* Includes exercises and solutions
More details
Series
Edition
2nd edition
Language
English
Place of publication
Bosa Roca
United States
Publishing group
Taylor & Francis Inc
Target group
College/higher education
Professional and scholarly
Illustrations
89 s/w Abbildungen, 3 farbige Abbildungen, 33 s/w Tabellen
33 Tables, black and white; 3 Illustrations, color; 89 Illustrations, black and white
Dimensions
Height: 254 mm
Width: 178 mm
Thickness: 16 mm
Weight
550 gr
ISBN-13
978-0-8153-4488-9 (9780815344889)
Copyright in bibliographic data and cover images is held by Nielsen Book Services Limited or by the publishers or by their respective licensors: all rights reserved.
Schweitzer Classification
Other editions
Additional editions

Philip Bonner
Protein Purification
E-Book
09/2018
2nd Edition
Routledge Cavendish
€100.99
Available for download

Philip Bonner
Protein Purification
E-Book
09/2018
2nd Edition
Routledge Cavendish
€100.99
Available for download

Philip Bonner
Protein Purification
Book
08/2018
2nd Edition
CRC Press
€253.20
Shipment within 10-20 days
Previous edition

Philip Bonner | Philip L.R. Bonner
Protein Purification
Book
02/2007
1st Edition
Taylor & Francis
€78.17
Article exhausted; check for reprint
Person
Dr Philip Bonner is Programme Leader for the MRes in Applied Biosciences at Nottingham Trent University.
Content
1. Water, pH and buffers. 2. Protein structure and properties. 3. Chromatography and the strategy of protein purification. 4. Groundwork. 5. Non-affinity absorption techniques used to purify proteins. 6. Affinity-based procedures used to purify proteins. 7. Non-absorption techniques for purifying proteins. 8. Methods for monitoring the purity of protein solutions. Appendix 1: The laws of thermodynamics and the Gibbs free energy equation. Appendix 2: Properties of amino acids. Appendix 3: The common units used in biology. Appendix 4: Chromatographic runs. Appendix 5: Determining the concentration of a compound in a solution in parts per million (ppm). Appendix 6: The answers to the exercises in the various chapters. Appendix 7: An alphabetical list of chromatography, electrophoresis, filtration equipment and laboratory suppliers. Appendix 8: Buffer tables to prepare at a required pH value.