Cell Culture Engineering VI

Cell therapy in kidney failure.- Improved bicistronic mammalian expression vectors using expression augmenting sequence element (EASE).- Effects on growth behavior in continuous hybridoma cell cultures: The role of viral contamination.- Isolation, characterization and recombinant protein expression in Veggie-CHO: A serum-free CHO host cell line.- Collective experiences of adventitious viruses of animal-derived raw materials and what can be done about them.- An overview of viral and viral-like agents in cell culture systems.- New adenovirus vectors for protein production and gene transfer.- Modulation of cell cycle progression and of antibody production in mouse hybridomas by a nucleotide analogue.- Engineering Chinese hamster ovary (CHO) cells to achieve an inverse growth - associated production of a foreign protein, ?-galactosidase.- A high-yielding serum-free, suspension cell culture process to manufacture recombinant adenoviral vectors for gene therapy.- Recombinant insulin-like growth factor-I (IGF-I) production in Super-CHO results in the expression of IGF-I receptor and IGF binding protein 3.- Attachment and growth of anchorage-dependent cells on a novel, charged-surface microcarrier under serum-free conditions.- Regulated multicistronic expression technology for mammalian metabolic engineering.- Design, characterization and application of a minibioreactor for the culture of human hematopoietic cells under controlled conditions.- Historical reflections on cell culture engineering.- Optimization of transient gene expression in mammalian cells and potential for scale-up using flow electroporation.- Population balance model of in vivo neutrophil formation following bone marrow rescue therapy.- Mammalian cell retention devides for stirred perfusion bioreactors.-Variable functions of bcl-2 in mediating bioreactor stress-induced apoptosis in hybridoma cells.- Apoptosis-resistant NS/0 E1B-19K myelomas exhibit increased viability and chimeric antibody productivity under cell cycle modulating conditions.- Effects of temperature on recombinant protein expression in Semliki Forest virus infected mammalian cell lines growing in serum-free suspension cultures.- Effects of CO2 and osmolality on hybridoma cells: growth, metabolism and monoclonal antibody production.- pQuattro vectors allow one-step multigene metabolic engineering and auto-selection of quattrocistronic artificial mammalian operons.