Basic Cloning Procedures
Published on 7. July 2012
Book
Paperback/Softback
VII, 169 pages
978-3-642-48977-8 (ISBN)
Description
This manual introduces the reader to bacic methods used in the isolation, cloning and analysis of genetic material. The protocols include RT-PCR amplification, gene cloning, hybridization analysis and sequencing of nucleic acids, PCR-based site-specific mutagenesis, analysis of protein DNA-specific interaction, cell-free protein synthesis and product electrophoretic and immunological analysis.
More details
Series
Edition
1998 ed.
Language
English
Place of publication
Berlin
Germany
Publishing group
Springer Berlin
Target group
Professional and scholarly
Research
Illustrations
VII, 169 p.
Dimensions
Height: 235 mm
Width: 155 mm
Thickness: 11 mm
Weight
283 gr
ISBN-13
978-3-642-48977-8 (9783642489778)
DOI
10.1007/978-3-642-71965-3
Schweitzer Classification
Other editions
Additional editions
Valdis Berzins
Basic Cloning Procedures
Book
03/1998
Springer
€85.55
Article exhausted; check different version
Content
1 cDNA Synthesis and Cloning.- 2 DNA Sequencing.- 3 RNA In Vitro Synthesis by Phage T7 DNA-Dependent RNA Polymerase.- 4 PCR-Based Site-Specific Mutagenesis.- 5 Analysis of Specific Protein-DNA Interactions.- 6 Gel Electrophoresis, Transfer and Hybridization of Nucleic Acids.- 7 Immunological Methods for Analysis of Recombinant Proteins.- 8 Protein Synthesis in Cell-Free Systems.