Basic Cloning Procedures
Published on 10. March 1998
Book
Spiral bound
VII, 169 pages
978-3-540-63539-0 (ISBN)
Description
This manual introduces the reader to basic methods used in the isolation, cloning and analysis of genetic material. The protocols include RT-PCR amplification, gene cloning, hybridization analysis and sequencing of nucleic acids, PCR-based site-specific mutagenesis, analysis of protein DNA-specific interaction, cell-free protein synthesis and product electrophoretic and immunological analysis. Each protocol includes short background information, a detailed description of the necessary materials, step-by-step procedures, a troubleshooting guide and useful practical hints.
More details
Series
Language
English
Place of publication
Heidelberg
Germany
Publishing group
Springer Berlin
Target group
Professional and scholarly
Illustrations
3 s/w Tabellen
3 black & white tables, biography
Dimensions
Height: 23.5 cm
Width: 15.5 cm
Weight
315 gr
ISBN-13
978-3-540-63539-0 (9783540635390)
DOI
10.1007/978-3-642-71965-3
Schweitzer Classification
Other editions
Additional editions
Content
Juris Steinbergs, Aleksander Tsimanis: cDNA Synthesis and Cloning.- Eriks Jankevics: DNA Sequencing. - Elita Avota, Normunds Licis: RNA in Vitro Synthesis by Phage T7 DNA-Dependent RNA Polymerase.- Janis Klovins, Valdis Berzins: PCR-Based Site-Specific Mutagenesis.- Indrikis Muiznieks, Nils Rostoks: Analysis of Specific Protein--DNA Interactions.- Illar Pata, Erkki Truve: Gel Electrophoresis, Transfer and Hybridization of Nucleic Acids.- Irina Sominskaya, Kaspars Tars: Immunological Methods for Analysis of Recombinant Proteins.- Tiina Tamm, Erkki Truve: Protein Synthesis in Cell-Free Systems.