Histological Analysis of Endocrine Disruptive Effects in Small Laboratory Fish
Published on 29. July 2009
Software
Other digital
360 pages
978-0-470-43179-5 (ISBN)
More details
Language
English
Place of publication
Hoboken
United Kingdom
Publishing group
John Wiley and Sons Ltd
Target group
Professional and scholarly
Dimensions
Height: 250 mm
Width: 150 mm
Thickness: 15 mm
Weight
666 gr
ISBN-13
978-0-470-43179-5 (9780470431795)
Copyright in bibliographic data and cover images is held by Nielsen Book Services Limited or by the publishers or by their respective licensors: all rights reserved.
Schweitzer Classification
Other editions
Additional editions
Daniel Dietrich | Heiko O. Krieger
Histological Analysis of Endocrine Disruptive Effects in Small Laboratory Fish
E-Book
08/2009
Wiley
€141.99
Available for download
Person
Daniel R. Dietrich, PhD , is a Professor and Head of the Human and Environmental Toxicology Group, Faculty of Sciences of the University of Konstanz, Konstanz, Germany. Heiko O. Krieger, MSc , is a scientific research assistant and PhD student in the Human and Environmental Toxicology Group, Faculty of Sciences of the University of Konstanz, Konstanz, Germany.
Content
Preface. Acknowledgments. Contributing Authors. 1 Introduction. References. 2 Fish Species of Interest. 2.1 Fathead Minnow (Pimephales promelas). 2.2 Medaka (Oryzias latipes). 2.3 Zebrafish (Danio rerio). 2.4 Other Fish Species. References. 3 Sexual Determination, Differentiation, and Gonadal Development. 3.1 Primordial Germ Cells in the Primordial (Primary) Gonad. 3.2 Reproductive Strategies. 3.3 Differentiation of the Primordial Gonad into Ovary or Testis. 3.4 Gonadal Duct Formation. 3.5 Endocrinology: Influence on Gonadogenesis. 3.6 Critical Period of Sexual Differentiation in Developing Fish. 3.7 Bi-Potentiality of Germ Cells in Adult Fish. References. 4 Female Gonad Anatomy and Morphology. 4.1 Gonadogenesis: Ovary. 4.2 Hypothalamic-Pituitary-Ovarian Axis. 4.3 Cellular Structure of the Ovary. References. 5 Male Gonad Anatomy and Morphology. 5.1 Gonadogenesis: Testes. References. 6 Endocrine-Disrupting Compounds. 6.1 Individual Effects. 6.2 Effects Associated with Exposure to Specific Compounds or Compound Classes. 6.3 Population Effects. References. 7 Determination of Effects of Exogenous Hormones and Endocrine-like Active Compounds. 7.1 Histological Processing: Microdissection versus Whole-Fish Sectioning. 7.2 Optimal Tissue Preparation and Histological Techniques. 7.3 Plane of Gonad Sectioning for Optimal Organ Representation. References. 8 Evaluation of Effects in Fish Gonads. 8.1 Qualitative (Semiquantitative) versus Quantitative Evaluation. 8.2 Gonadal Staging in the Testis. 8.3 Gonadal Staging in the Ovary . 8.4 Qualitative Assessment of Histopathological Changes. References. 9 Experimental Design and Statistics. 9.1 Basic Considerations in Experimental Design. 9.2 Variables to be Determined and Their Inherent Biological and Mathematical Characteristics. 9.3 Prerequisite Statistical Concepts. 9.4 Statistical Tests and Testing Situations Encountered Routinely. References. 10 Conclusions. References. Appendix: Fish Preparation and Microdissection of Organs. A.1 Fish Preparation. A.2 Microdissection of Organs. A.3 Tissue Fixation. A.4 Embedding. A.5 Tissue Sectioning. A.6 Sample Mounting. A.7 Tissue Slide Staining. A.8 Final Processing. A.9 Examples. A.10 Summary. References. Index.