The Zebrafish: Genetics, Genomics, and Transcriptomics

Part 1. Forward and Reverse Genetics1. Multiplex Conditional Mutagenesis in Zebräsh Using the CRISPR/Cas System2. Tol2-Mediated Transgenesis, Gene Trapping and Enhancer Trapping, and the Gal4-UAS System3. Genetic Screens for Mutations Affecting Adult Traits and Parental-Effect Genes4. Targeted Candidate Gene Screens Using CRISPR/Cas9 Technology5. TALEN and CRISPR Enhanced DNA Homologous Recombination for Gene Editing in Zebrafish6. Precise Genome Editing by Homologous Recombination7. The Zebrafish Genome Editing Toolkit8. Clonal Analysis of Gene Loss-of-function and Tissue-specific Gene Deletion in Zebrafish via CRISPR/Cas9 Technology9. Tissue-Specific Gene Targeting Using CRISPR/Cas9 Part 2. Transgenesis and Functional Genomics10. Transcriptional Regulation Using the Q System in Transgenic Zebrafish11. Contemporary Zebrafish Transgenesis with Tol2 and Application for Cre/lox Recombination Experiments12. Method for Somatic Cell Nuclear Transfer in Zebrafish13. Experimental Approaches to Studying the Nature and Impact of Splicing Variation in Zebrafish14. PICCORO: A Technique for Manipulating the Activity of Transcription Factors with Blue Light Part 3. Transcriptomics15. Tomo-seq: A Method to Obtain Genome-Wide Expression Data with Spatial Resolution16. Cell-Type Specific Transcriptomic Analysis by Thiouracil Tagging in Zebrafish17. RT-qPCR Gene Expression Analysis in Zebrafish: Preanalytical Precautions and Use of Expressed Repetitive Elements (ERE) for Normalization Part 4. Epigenetics18. Genome-Wide DNA Methylation Profiling in Zebrafish19. Histone Modifications in Zebrafish Development20. Chromatin Immunoprecipitation and an Open Chromatin Assay in Zebrafish Erythrocytes21. Assay for Transposase-Accessible Chromatin and Circularized Chromosome Conformation Capture, Two Methods to Explore the Regulatory Landscapes of Genes in Zebrafish22. Epigenetic Regulation of Hematopoietic Stem Cell Development Part 5. Zebrafish Infrastructure23. A Scientist's Guide for Submitting Data to ZFIN24. New Frontiers for Zebrafish Management25. Aquaculture, Husbandry, and Shipping at the Zebrafish International Resource Center26. Health Monitoring and Disease Prevention at the Zebrafish International Resource Center