The Zebrafish: Genetics, Genomics and Informatics

Part I: Forward and Reverse Genetics 1. Gene Trap Mutagenesis for Generation of Conditional Mutations in Zebrafish - Wenbiao Chen 2. Advances in Tol2 Transposon Mutagenesis - Koichi Kawakami 3. OPEN Zinc Finger Nucleases - Keith Joung and Randy Peterson 4. Retroviral-Mediated Insertional Mutagenesis in Zebrafish - Adam Amsterdam, Nancy Hopkins, and Shawn Burgess 5. Genetic Screens for Maternal-Effects Mutations - Francisco Pelegri and Mary Mullins 6. High-Throughput Target-Selected Gene Inactivation in Zebrafish - Derek Stemple 7. Suppressor Screens in Haploids - Xiaoying Bai 8. Transposons - Stephen Ekker 9. Caged MOs - James Chen Part II: Transgenesis 1. Advanced Transgenesis with Cre Recombinase - Christian Mosimann 2. Transgenesis with phiC31 Recombinase - James Lister 3. Nuclear Transfer - Jose Cibelli and Dr. Siripattarapravat Part III: The Zebrafish Genome and Mapping Technologies 1. The Zebrafish Genome Project: Sequence Analysis and Annotation- Derek Stemple 2. Molecular Cytogenetic Methodologies and a Bacterial Artificial Chromosome (BAC) Probe Panel Resource for Genomic Analyses in Zebrafish - Charles Lee and Amanda Smith 3. Automated Analysis of Conserved Syntenies for the Zebrafish Genome - John Postlethwait, Julian Catchen 4. The Zon Laboratory Guide to Positional Cloning in Zebrafish -Yi Zhou and Leonard Zon Part IV: Informatics and Comparative Genomics 1. Data Warehouse ZFIN - Monte Westerfield and Judy Sprague 2. Epigenetics: Methylation - Brad Cairns 3. Chromatin Structure - Leonard Zon and Yi Zhou 4. ChipSeq - Brad Cairns 5. Micro RNAs - Graham Lieshke and Luke Pase 6. Microarrays - Yi Zhou 7. Sequence-Based Transcriptional Profiling - Shawn Burgess 8. Epigenetic Regulation of Zebrafish Development - Elke Ober and co-author Part V: Infrastructure 1. Zebrafish Facilities for Small and Large Laboratories - Christian Lawrence 2. Genetic Backgrounds, Standard Lines, and their Husbandry - ZIRC, Zoltan