The Zebrafish: Cellular and Developmental Biology
Cellular and Developmental Biology
Academic Press
2nd Edition
Published on 7. December 2004
656 pages
978-0-08-047345-1 (ISBN)
Description
This volume of Methods in Cell Biology, the first of two parts on the subject of zebrafish, provides a comprehensive compendia of laboratory protocols and reviews covering all the new methods developed since 1999. This first volume provides state-of-the-art descriptions of novel cellular imaging technologies and methods for culture of zebrafish stem cells, summarizes protocols for analyzingthe development of major organ systems including the central nervous system (CNS), and introduces the use of the zebrafish as a model system for human diseases.
- Details state-of-the art zebrafish protocols, delineating critical steps in the procedures as well as potential pitfalls
- Illustrates many techiques in full-color
- Summarizes the Zebrafish Genome Project
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H. William Detrich | Leonard I. Zon | Monte Westerfield
The Zebrafish: v.76
Cellular and Developmental Biology
Book
11/2004
2nd Edition
Academic Press
€117.61
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Content
- Front Cover
- The Zebrafish: Cellular and Developmental Biology
- Copyright Pagae
- Contents
- Contributors
- Preface
- Dedication
- Part I: Cell Biology
- Chapter 1. Use of the DsRed Fluorescent Reporter in Zebrafish
- I. Introduction
- II. DsRed: History and Properties
- III. DsRed in Transgenic Zebrafish
- IV. Use of Multiple Fluorescent Reporters in Transplantation Assays
- V. Fusion Protein Reporters
- VI. Conclusion
- References
- Chapter 2. Analyzing Axon Guidance in the Zebrafish Retinotectal System
- I. Introduction
- II. Retinotectal Mutants
- III. Labeling the Retinotectal System
- IV. Perturbing the Retinotectal System
- V. Future Directions
- References
- Chapter 3. Optical Projection Tomography for Spatio-Temporal Analysis in the Zebrafish
- I. Introduction
- II. The Principle of Tomography
- III. Problems for OPT in Zebrafish
- IV. Materials
- V. Methods
- VI. Sample Preparation
- VII. Scanning
- VIII. Reconstruction
- IX. Presentation of Reconstructions
- X. Discussion
- XI. The Future of OPT
- XII. OPT Equipment
- References
- Chapter 4. Imaging Blood Vessels in the Zebrafish
- I. Introduction
- II. Imaging Vascular Gene Expression
- III. Nonvital Blood Vessel Imaging
- IV. Vital Imaging of Blood Vessels
- V. Conclusion
- References
- Chapter 5. Zebrafish Apoptosis Assays for Drug Discovery
- I. Introduction
- II. Zebrafish Apoptosis Genes
- III. Caspase Substrate Recognition in Zebrafish
- IV. Detection of Apoptosis In Situ
- V. Perspectives
- References
- Chapter 6. Lipid Metabolism in Zebrafish
- I. Introduction
- II. Genetic Screen-Forward, Reverse, and Targeted-Mutagenesis
- III. Pharmacological Studies Using Zebrafish
- IV. Mechanism of Action Studies Using Zebrafish. The Annexin2-Caveolin1 Complex
- V. Gene-Specific Studies: The Acyl-CoA Synthetase Enzymes
- VI. Summary
- References
- Chapter 7. Analysis of the Cell Cycle in Zebrafish Embryos
- I. Introduction: The Cell Cycle in Zebrafish
- II. Zebrafish Embryo Cell Cycle Protocols
- III. Screening for Mutations Affecting the Zebrafish Cell Cycle
- IV. Screening for Chemical Suppressors of Zebrafish Cell Cycle Mutants
- V. Conclusions
- VI. Reagents and Supplies
- References
- Chapter 8. Cellular Dissection of Zebrafish Hematopoiesis
- I. Introduction
- II. Zebrafish Hematopoiesis
- III. Hematopoietic Cell Transplantation
- IV. Enrichment of Hematopoietic Stem Cells
- V. Conclusions
- References
- Chapter 9. Culture of Embryonic Stem Cell Lines from Zebrafish
- I. Introduction
- II. Methods
- III. Materials
- References
- Part II: Developmental and Neural Biology
- Chapter 10. Neurogenesis
- I. Introduction
- II. The Primary Neuronal Scaffold
- III. Early Development of the Zebrafish Neural Plate
- IV. Lateral Inhibition and the Neurogenesis Cascade
- V. Establishment of Neuronal Identity
- VI. Aspects of Neuronal Differentiation
- VII. Useful Tools for the Study of Zebrafish Neurogenesis
- VIII. Conclusion
- References
- Chapter 11. Time-Lapse Microscopy of Brain Development
- I. Introduction-Why and When to Use Intravital Imaging
- II. Techniques for Vital Staining of the Nervous System
- III. Preparation of the Zebrafish Specimen
- IV. The Microscopic System
- V. Data Recording
- VI. Data Analysis
- VII. Pitfalls to Avoid
- VIII. Summary
- References
- Further Reading
- Chapter 12. Development of the Peripheral Sympathetic Nervous System in Zebrafish
- I. Introduction
- II. The Peripheral Autonomic Nervous System
- III. The Zebrafish as a Model System for Studying PSNS Development
- IV. Zebrafish as a Novel Model for Studying Neuroblastoma
- V. Conclusion and Future Directions
- References
- Chapter 13. Optical Physiology and Locomotor Behaviors of Wild-Type and Nacre Zebrafish
- I. Introduction
- II. Optical Physiology-Basics
- III. Optical Physiology-Alternative Approaches
- IV. Options for Improving the Transparency of Larval Zebrafish
- V. Behavioral Perturbation Studies
- VI. Conclusions
- References
- Chapter 14. Computer-Aided Screening for Zebrafish Embryonic Motility Mutants
- I. Introduction
- II. Flatbed Transparency Scanners and Computers
- III. Software
- IV. Embryos and Larva: Physiological Considerations
- V. Data Acquisition
- VI. Data Analysis
- VII. Potential Problems and Remedies
- VIII. Conclusions
- References
- Chapter 15. Photoreceptor Structure and Development: Analyses Using GFP Transgenes
- I. Introduction
- II. Photoreceptor Development
- III. Transgenic Zebrafish Expressing Photoreceptor-Specific Reporter Genes
- IV. Developmental Studies of Photoreceptors Using Transgenic Zebrafish
- V. Conclusions
- References
- Chapter 16. Approaches to Study Neurogenesis in the Zebrafish Retina
- I. Introduction
- II. Development of the Zebrafish Retina
- III. Analysis of Wild-Type and Mutant Zebrafish Visual Systems
- IV. Genetic Analysis of the Zebrafish Retina
- V. Summary
- References
- Chapter 17. Instrumentation for Measuring Oculomotor Performance and Plasticity in Larval Organisms
- I. Introduction
- II. Methods
- III. Results and Discussion
- IV. Conclusion
- V. Supplemental Movie Descriptions
- References
- Chapter 18. Development of Cartilage and Bone
- I. Introduction
- II. Cartilage Visualization Techniques
- III. Bone Visualization Techniques
- IV. Molecular Markers of Skeletal Precursors
- V. Strategy and Potential of Future Screens for Skeletal Mutants
- References
- Chapter 19. Morphogenesis of the Jaw: Development Beyond the Embryo
- I. Larval Zebrafish Craniofacial Cartilage Development
- II. Analysis of Craniofacial Skeletal and Replacement Tooth Development
- III. Conclusion
- References
- Chaptesr 20. Cardiac Development
- I. Introduction
- II. Stages of Heart Tube Morphogenesis
- III. Gene Expression
- IV. Conclusion and Future Directions
- References
- Chapter 21. Chemical Approaches to Angiogenesis
- I. Introduction
- II. Screening Tools
- III. Chemical Screening
- IV. Sensitized Screens
- References
- Chapter 22. Vascular Occlusion and Thrombosis in Zebrafish
- I. Introduction
- II. Vascular Occlusion
- III. Methods
- IV. Future Perspectives
- References
- Chapter 23. Zebrafish Kidney Development
- I. Introduction
- II. Pronephric Structure and Function
- III. Pronephric Development
- IV. The Zebrafish Pronephros as a Model of Human Disease
- V. Conclusions
- References
- Chapter 24. Pancreas Development in Zebrafish
- I. Pancreas Organogenesis
- II. Morphology of the Mature Pancreas
- III. Physiology of the Endocrine Pancreatic Tissues
- IV. Pancreas Development
- V. Formation and Patterning of the Zebrafish Endoderm
- VI. Formation of the Pancreatic Primordium
- VII. Cell Type Specification and Differentiation of the Endocrine Pancreas
- VIII. Formation of the Exocrine Pancreas
- IX. Perspectives
- References
- Part III: Disease Models
- Chapter 25. Mosaic Eyes, Genomic Instability Mutants, and Cancer Susceptibility
- I. Introduction
- II. Mutagenesis and Screening for Mosaic Eyes
- III. Mosaic Eyes. Genomic Instability or Retinal Mutations?
- IV. Studying Cancer Susceptibility in Adult Zebrafish
- V. Transgenic Analysis of Genomic Instability
- VI. Summary
- References
- Chapter 26. Discovery and Use of Small Molecules for Probing Biological Processes in Zebrafish
- I. Rationale for Small Molecule Screens in Zebrafish
- II. Assay Development
- III. Chemical Suppressor and Enhancer Screens
- IV. Selection of Small Molecule Libraries
- V. Screening Methods
- VI. Follow-Up Studies for Active Small Molecules
- VII. Conclusions
- References
- Chapter 27. Modeling Human Disease by Gene Targeting
- I. Introduction
- II. DNA Repair Processes
- III. Transient Means to Effect Altered Gene Expression in the Zebrafish
- IV. Heritable Means to Effect Altered Gene Expression in the Zebrafish
- V. Discussion
- References
- Index
- Volumes in Series
