Yeast Cells
Academic Press
Published on 22. January 1976
394 pages
978-0-08-085909-5 (ISBN)
Description
Yeast Cells
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D.Marshall Prescott
Methods in Cell Biology: v. 12
Book
12/1975
Academic Press
€136.40
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Content
- Cover
- Contributors to This Volume
- Contents
- List of Contributors
- Preface
- Chapter 1. Growth and Handling of Yeasts
- I. Introduction
- II. Isolation and Maintenance of Yeasts
- III. Growth of Yeasts
- IV. Procedures Used in Handling Yeast Populations
- References
- Chapter 2. Inhibitors of Macromolecular Synthesis in Yeast
- I. Introduction
- II. Inhibitors
- III. Effects of Inhibitors on Yeast Metabolism
- IV. Conclusion
- References
- Chapter 3. Isolation of Yeast DNA
- I. Introduction
- II. Isolation Procedure
- References
- Chapter 4. Preparation of RNA and Ribosomes from Yeast
- I. Introduction
- II. Cell Growth and Disruption
- III. Radioactive Labeling
- IV. Preparation of Ribosomes
- V. Preparation of Ribosomal Subunits
- VI. Preparation of Polyribosomes
- VII. Preparation of High-Molecular-Weight rRNAs
- VIII. Preparation of Low-Molecular-Weight RNAs
- IX. Polyacrylamide Gel Electrophoresis of Low-Molecular-Weight RNAs
- X. High-Molecular Weight rRNA Precursors
- XI. mRNA
- References
- Chapter 5. DNA-Dependent RNA Polymerases from Yeasts
- I. Introduction
- II. Purification Procedures
- III. Characterization of the RNA Polymerases of Yeasts
- IV. Preparation of Yeast DNA Active as Template for Yeast RNA Polymerase
- V. Discussion
- References
- Chapter 6. The Isolation of Yeast Nuclei and Methodr to Study Their Properties
- I. Introduction
- II. Isolation of Nuclei from Yeast Protoplasts
- III. Isolation of Nuclei from Normal Yeast Cells
- IV. Selection of a Method for the Isolation of Yeast Nuclei
- V. Preparation of a Dense Crescent (Nucleolar) Fraction from Yeast Nuclei
- VI. Electron Microscopy of Isolated Yeast Nuclei
- VII. Isolation and Characterization of Chromatin from Yeast Nuclei
- VIII. Isolation and Characterization of DNA from Yeast Nuclei
- IX. Isolation and Characterization of RNA from Yeast Nuclei
- X. Isolation and Characterization of Nuclear Proteins
- XI. Yeast Nuclear Enzymes
- XII. Concluding Remarks
- References
- Chapter 7. Isolation of Vacuoles from Yeasts
- I. Introduction
- II. Preparation of Spheroplasts
- III. Extraction of Intact Vacuoles from Spheroplasts
- IV. Characterization of Isolated Vacuoles
- Note Added in Proof
- References
- Chapter 8. Analytical Methods for Yeasts
- I. Introduction
- II. Macromolecular Components of Yeast Cells
- III. Small-Molecule Pools in Yeasts
- IV. Lipids
- V. Concluding Remarks
- References
- Chapter 9. Methods for Avoiding Proteolytic Artefacts in Studies of Enzymes and Other Proteins from Yeasts
- I. Introduction
- II. Known Cases of Proteolytic Artefacts in Yeast Studies
- III. The Yeast Proteases
- IV. Antiartefact Methodology
- V. Concluding Remarks
- References
- Chapter 10. Induction of Haploid Glycoprotein Mating Factors in Diploid Yeasts
- I. Biology of the Yeast Hansenula wingei
- II. Methods
- III. Control of Haploid Mating Function in the Diploid
- IV. Induction of 5f in the Diploid
- V. Induction of 21f in the Diploid
- VI. Significance of Induction of Sex-Specific Cell Surface Glycoproteins in the Diploid
- References
- Chapter 11. Mutagenesis in Yeast
- I. Introduction
- II. Forward Mutations
- III. The Detection of Reverse Mutations
- IV. Lethal Mutations
- V. Molecular Mutagensis
- VI. The Mutation Process
- VII. Special Problems
- References
- Chapter 12. Induction, Selection, and Experimental Uses of Temperature-Sensitive and Other Conditional Mutants of Yeast
- I. Introduction
- II. Induction of Mutants
- III. Isolation of Mutants
- IV. Handling and Preservation of Strains
- The Problem of Genetic Complexity
- V. Experimental Uses of Temperature-Sensitive (ts) Mutants
- VI. Other Types of Conditional Mutants
- References
- Chapter 13. In Vivo and in Vitro Synthesis of Yeast Mitochondrial DNA
- I. Introduction
- II. In Vivo Synthesis
- III. In Vitro Synthesis
- IV. Extraction and Analysis of mtDNA
- V. Examples of Results
- VI. Concluding Remarks
- References
- Chapter 14. Isolation of Mitochondria and Techniques for Studying Mitochondrial Biogenesis in Yeasts
- I. Introduction
- II. Growth Determinants of Mitochondrial Structure and Activity
- III. Yeast Culture Conditions for Mitochondrial Isolation
- IV. Isolation of Mitochondria
- V. General Techniques for the Study of Mitochondrial Biogenesis
- References
- Chapter 15. Separation and Some Properties of the Inner and Outer Membranes of Yeast Mitochondria
- I. Introduction
- II. Membrane Separation
- III. Discussion
- References
- Chapter 16. The Use of Fluorescent DNA-Binding Agent for Detecting and Separating Yeast Mitochondria DNA
- I. Introduction
- II. The Use of DAPI in Cesium Chloride Gradients
- III. Fluorescent Staining of Cells with DAPI
- IV. Conclusion
- References
- Chapter 17. Cytoplasmic Inheritance and Mitochondrial Genetics in Yeasts
- I. Introduction
- II. The Petite Mutation
- III. Genetics of the Petite Mutation
- IV. Genetics of Mitochondrial Resistance to Antibiotics
- V. Recombination of Mitochondrial Factors
- VI. Aspects of Mitochondrial Transmission
- VII. Aspects of Mitochondrial Recombination
- VIII. The Petite Mutation and Mapping of Mitochondrial Genes
- IX. Mitochondrial Markers
- X. Concluding Remarks on Mitochondrial Genetics
- XI. Tetracycline Resistance
- XII. Omicron DNA
- XIII. The Psi Factor
- XIV. The Killer Character in Yeasts
- XV. Cytoplasmic Mutagens
- XVI. Summary
- References
- Chapter 18. Synchronization of the Fission Yeast Schizosaccharomyces pombe Using Heat Shocks
- I. Introduction
- II. Media and Cultures
- III. The Cyclic Thermostat
- IV. The Synchronizing Procedure
- V. Conclusion
- References
- Index
- Contents of Previous Volumes
