Yeast Cells
Academic Press
Published on 17. December 1975
345 pages
978-0-08-085908-8 (ISBN)
Description
Yeast Cells
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D.Marshall Prescott
Methods in Cell Biology: v. 11
Book
10/1975
Academic Press
€136.40
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Content
- Cover
- Contributors
- Contents
- List of Contributors
- Preface
- Chapter 1. The Preparation of Yeasts for Light Microscopy
- I. Light Microscopy of Living Yeasts
- II. Fixed and Stained Preparations
- III. Summary and Cautionary Remarks
- References
- Chapter 2. Electron Microscopy of Yeasts
- I. General Remarks
- II. Fixation and Staining
- III. Freeze-Fracturing and Freeze-Etching, Interpretation
- References
- Chapter 3. Methods in Sporulation and Germination of Yeasts
- I. Sporulation
- II. Germination
- References
- Chapter 4. Synchronous Mating in Yeasts
- I. Introduction
- II. Mating Procedures
- Ill. Discussion
- References
- Chapter 5. Synchronous Zygote Formation in Yeasts
- I. General Characteristic of the Conjugation Process
- II. Basic Principles for Synchronous Mass Production of zygotes
- III. Method for Poorly Synchronized Zygote Formation for Genetic Analysis
- IV. Method of Synchronous Zygote Mass Production
- V. Limitations of the Method
- References
- Chapter 6. Continuous Cultivation of Yeasts
- I. Introduction
- II. Homocontinuous Single-Stage Systems
- III. Cultivation of Glucose-Insensitive Yeast Types
- IV. Cultivation of Glucose-Sensitive Yeast Types
- V. Multistage Systems
- VI. Concluding Remarks
- References
- Chapter 7. Methods for Monitoring the Growth of Yeast cultures and for Dealing with the Clumping Problem
- I. Introduction
- II. Stopping Growth Rapidly
- III. Measuring Biomass and Its Components
- IV. Dealing with the Clumping Problem and Measuring Cytokinesis
- V. Measuring the Number of Cells
- VI. Measuring Turbidity
- VII. Taking Advantage of the Budding Mode of Reproduction
- VIII. Monitoring for Contamination
- References
- Chapter 8. Preparation and Growth of Yeast Protoplasts
- I. Introduction
- II. Preparation of Yeast Protoplasts
- III. Factors Affecting the Growth and Metabolic Activities of Protoplasts
- IV. Growth and Macromolecular Synthesis
- References
- Chapter 9. Dissecting Yeast Asci without a Micromanipulator
- References
- Chapter 10. Use of Micromanipulators in Yeast Studies
- I. Introduction
- II. Apparatus
- III. Micromanipulation
- References
- Chapter 11. Cell cycle Analysis
- I. Introduction
- II. Single-Cell Techniques
- III. Synchronous Cultures
- IV. Conclusions
- References
- Chapter 12. Genetic Mapping in Yeast
- I. Introduction
- II. Mapping Techniques
- III. Genetic Map
- References
- Chapter 13. The Use of Mutants in Metabolic Studies
- I. Introduction
- II. Selection and Conservation of Mutants
- III. Elucidation of a Metabolic Pathway
- IV. Study of Pathway Interactions
- V. Study of Regulatory Phenomena
- VI. Conclusion
- References
- Chapter 14. Isolation of Regulatory Mutants in Saccharomyces cerevisiae
- I. Introduction
- II. Arginine
- III. Galactose Degradation
- IV. Acid Phosphatase Biosynthesis
- V. Pyrimidine Biosynthesis
- VI. Methionine Biosynthesis
- VII. Histidine Biosynthesis
- References
- Chapter 15. Methods for Selecting Auxotrophic and Temperature-Sensitive Mutants in Yeasts
- I. Introduction
- II. Nystatin Selection
- III. Tritium Suicide Selection
- IV. Fatty Acid-less Death in S. cerevisiae
- References
- Chapter 16. Isolation and Characterization of Mutants of Saccharomyces cerevisiae Able to Grow after Inhibition of dTMP Synthesis
- I. Introduction
- II. Mutants Able to Grow after Inhibition of dTMP Synthesis
- III. Characterization of typ or tup Mutants
- IV. Isolation of dTMP Low Requirers (typ tlr Mutants)
- V. Mutants Auxotrophic for dTMP (typ tmp Mutants)
- VI. dTMP Auxotrophs with a Low Requirement for dTMP
- References
- Chapter 17. Mutants of Saccharomyces cerevisiae That Incorporate Deoxythymidine 5'-Monophosphate into DNA in Vivo
- I. Introduction
- II. Materials
- III. Isolation of Mutants Incorporating dTMP into DNA in Vivo
- IV. Incorporation of Nucleotides by tup Mutants
- V. Genetic Analysis
- VI. Applications
- References
- Chapter 18. Mutants of Meiosis and Ascospore Formation
- I. Introduction
- II. Isolation of Mutants
- III. Genetic Characterization of Mutants
- IV. Analysis of Mutant Phenotypes
- V. Summary
- References
- Index
- Contents of Previous Volumes
