An Introduction to Molecular Biotechnology
Fundamentals, Methods and Applications
2nd Edition
Published on 14. November 2013
XXXIV, 602 pages
978-3-527-67203-5 (ISBN)
Description
Molecular biotechnology continues to triumph, as this textbook testifies - edited by one of the academic pioneers in the field and written by experienced professionals. This completely revised second edition covers the entire spectrum, from the fundamentals of molecular and cell biology, via an overview of standard methods and technologies, the application of the various "-omics", and the development of novel drug targets, right up to the significance of system biology in biotechnology. The whole is rounded off by an introduction to industrial biotechnology as well as chapters on company foundation, patent law and marketing.
The new edition features:
- Large format and full color throughout
- Proven structure according to basics, methods, main topics and economic perspectives
- New sections on system biology, RNA interference, microscopic techniques, high throughput sequencing, laser applications, biocatalysis, current biomedical applications and drug approval
- Optimized teaching with learning targets, a glossary containing around 800 entries, over 500 important abbreviations and further reading.
The only resource for those who are seriously interested in the topic.
Bonus material available online free of charge: www.wiley-vch.de/home/molecbiotech
The new edition features:
- Large format and full color throughout
- Proven structure according to basics, methods, main topics and economic perspectives
- New sections on system biology, RNA interference, microscopic techniques, high throughput sequencing, laser applications, biocatalysis, current biomedical applications and drug approval
- Optimized teaching with learning targets, a glossary containing around 800 entries, over 500 important abbreviations and further reading.
The only resource for those who are seriously interested in the topic.
Bonus material available online free of charge: www.wiley-vch.de/home/molecbiotech
More details
Other editions
Additional editions
Book
03/2011
2nd Edition
Wiley-Blackwell
€91.90
Article exhausted; check for reprint
Person
Michael Wink studied biology and chemistry in Bonn and was awarded his doctorate from TU Braunschweig in 1980. After gaining his lecturing qualification in 1984/1985, he was awarded a Heisenberg grant by the German Research Council to work at the Max Planck Institute for Breeding Research in Cologne and from then at the gene center of Ludwig-Maximilians University in Munich. Following a chair for pharmaceutical biology at Mainz University in 1988, he accepted the post of Professor for Pharmaceutical Biology at the University of Heidelberg one year later. Between 2002 and 2004 he was the founding and managing director of the Institute for Pharmacy and Molecular Biotechnology, and dean from 2001 to 2005 for the new and popular course in molecular biotechnology offered by the university. Professor Wink's areas of interest include pharmaceutical research, molecular biotechnology, and medicinal plants, as well as research into secondary materials and evolution.
Content
FUNDAMENTALS OF CELLULAR AND MOLECULAR BIOLOGY
The Cell as the Basic Unit of Life (M. Wink)
Structure and Function of Cellular Macromolecules (M. Wink)
Structure and Functions of a Cell (M. Wink)
Biosynthesis and Function of Macromolecules (DNA, RNA, and Proteins) (M. Wink)
Distributing Proteins in the Cell (Protein Sorting) (M. Wink)
Evolution and Diversity of Organisms (M. Wink)
STANDARD METHODS IN MOLECULAR BIOTECHNOLOGY
Isolation and Purification of Proteins (T. Wieland, M. Lutz)
Peptide and Protein Analysis with Electrospray Tandem Mass Spectrometry (A. Schlosser, W.D. Lehmann)
Isolation of DNA and RNA (H. Weiher, R. Zwacka, I. Herr)
Chromatography and Electrophoresis of Nucleic Acids (H. Weiher, R. Zwacka, I. Herr)
Hybridization of Nucleic Acids (H. Weiher, R. Zwacka, I. Herr)
Use of Enzymes in the Modification of Nucleic Acids (A. Groth, R. Zwacka, H. Weiher, I. Herr)
Polymerase Chain Reaction (A. Mohr, H. Weiher, I. Herr, R. Zwacka)
DNA Sequencing (R. Zwacka, A. Mohr, I. Herr, H. Weiher)
Cloning Procedures (T. Wieland, S. Lutz)
Expression of Recombinant Proteins (T. Wieland, S. Lutz)
Patch Clamp Method (R. Kraft)
Cell Cycle Analysis (S. Wölfl, A. Kitanovic)
Microscopic Techniques (S. Diekmann)
Laser Applications (M. Vogel, R. Fink)
KEY TOPICS
Genomics and Functional Genomics (S.Wiemann, M. Frohme)
Bioinformatics (B. Brors, K. Fellenberg)
Cellular Systems Biology (H. Schmidt-Gienewinkel, S. Legewie, B. Brors, R. König)
Protein?Protein and Protein?DNA Interaction (P. Uetz, E. Pohl)
Drug Research (M. Koegl, R. Tolle, U. Deuschle, C. Kremoser)
Drug Targeting and Prodrugs (G. Fricker)
Molecular Diagnostics in Medicine (S. Wölfl, R. Gessner)
Recombinant Antibodies and Phage Display (S. Dübel)
Transgenic and Gene-Targeted Mice and their Impact in Medical Research (R. Sprengel)
Gene Therapy: Strategies and Vectors (A. Groth, I. Herr)
RNA Interference, Modified DNA, Peptide Nucleic Acid, and Applications in Medicine and Biotechnology (N. Metzler-Nolte, A. Sosniak)
Plant Biotechnology (H. Hillebrand, R. Hell)
Biocatalysis in the Chemical Industry (M. Breuer, B. Hauer)
BIOTECHNOLOGY IN INDUSTRY
Industrial Application: Biotech Industry, Markets, and Opportunities (J. Schüler)
Patents in the Molecular Biotechnology Industry: Legal and Ethical Issues (David B. Resnik)
Drug Approval in the European Union and United States (G. Walsh)
Emergence of a Biotechnology Industry (C. Kremoser)
The 101 of Founding a Biotech Company (C. Kremoser)
Marketing (C. Kremoser)
Further Reading
Abbreviations
Glossary (M. Wink)
Subject Index
1 Å =0.1 nm aa-tRNA aminoacyl-tRNA AAV adeno-associated virus ABC ATP binding cassette Acetyl-CoA acetyl coenzyme A AcNPV Autographa californica nuclear polyhedrosis virus ACRS amplification-created restriction sites ACTH adrenocorticotropic hormone ADA adenosine deaminase ADEPT antibody-directed enzyme pro-drug therapy ADME-T absorption, distribution, metabolism, excretion and toxicity ADP adenosine diphosphate ADRs adverse drug reactions AEC aminoethylcysteine AFLP amplified fragment length polymorphism AFM atomic force microscope AIDS acquired immune deficiency syndrome ALS amyotrophic lateral sclerosis AMP adenosine monophosphate AMPA α-amino-3-hydroxyl-5-methyl-4-isoxazol-propionate Ampr ampicillin resistance gene AMV avian myeloblastosis virus ANN artificial neural network AO acridine orange AOX1 alcohol oxidase 1 APC anaphase promoting complex ApoB100 apolipoprotein B100 ApoE apolipoprotein E APP amyloid precursor protein ARMS amplification refractory mutation system ARS autonomously replicating sequence ATP adenosine triphosphate att attachment site BAC bacterial artificial chromosome bcl2 B-cell leukemia lymphoma 2 (protein protecting against apoptosis) BfArM German Bundesinstitut für Arzneimittel und Medizinprodukte β-Gal β-galactosidase BHK-21 baby hamster kidney cells BLA biologics licence application BLAST basic local alignment search tool BMP bone morphogenetic proteins bp base pairs BrdU bromodeoxyuridine CA correspondence analysis CAD coronary artery disease CaM-Kinase Ca2+/calmodulin-dependent protein kinase cAMP cyclic AMP cap AAV gene mediating encapsulation CARS coherent anti-Raman scattering CAT Committee for Advanced Therapies CBER Center for Biologics Evaluation and Research CC chromatin remodelling complex CCD charge-coupled device CDER Center for Drug Evaluation and Research CDK cyclin-dependent kinase cDNA copy DNA CDR complementary determining region CDRH Center for Devices and Radiological Health CEO chief executive officer CFP cyan fluorescent protein CFTR cystic fibrosis transmembrane regulator CGAP cancer genome anatomy project CGH comparative genome hybridization CHMP Committee for Medicinal Products for Human Use CHO Chinese hamster ovary CIP calf intestinal phosphatase CML chronic myeloic leukemia CMN Corynebacterium-Mycobacterium-Nocardia group CMV cauliflower mosaic virus CMV Cytomegalovirus CNS central nervous system COMP Committee on Orphan Medicinal Products COS-1 simian cell line, CV-1, transformed by origin-defective mutant of SV40 cpDNA chloroplast DNA CPMV cowpea mosaic virus cPPT-sequence central polypurine tract – regulatory element in lentiviral vectors that facilitates double strand synthesis and the nuclear import of the pre-integration complex CSF colony-stimulating factor CSO contract service organisation CTAB cetyltrimethylammonium bromide CVM Center for Veterinary Medicine CVMP Committee for Medicinal Products for Veterinary Use 2D two-dimensional Da Dalton DAG diacylglycerol DAPI 4,6-diamidino-2-phenylindole dATP deoxyadenosine triphosphate DBD DNA-binding domain DAC divide-and-conquer strategy DD differential display DDBJ DNA Data Bank of Japan ddNTP dideoxynucleotide triphosphate DEAE diethylaminoethyl dHPLC denaturing HPLC DIC differential interference contrast DIP Database of Interacting Proteins DNA deoxyribonucleic acid DNAse deoxyribonuclease dNTP deoxynucleoside triphosphate Dox doxycycline ds diabodies disulfide-stabilized diabodies dsDNA double-stranded DNA dsFv-fragment disulfide-stabilized Fv fragment dsRNA double-stranded RNA DtxR diphtheria toxin repressor Ebola-Z envelope protein of the Ebola-Zaire virus, which has a high affinity to lung epithelial cells EC50 effective concentration, the dose or concentration that produces a 50% effect in the test population within a specified time ECD electron capture dissociation EDTA ethylenediaminetetraacetic acid ee enantiomeric excess EF2 elongation factor 2 EF-Tu elongation factor Tu EGF epidermal growth factor EGFP enhanced green fluorescent protein EGTA ethyleneglycol-bis-(2-aminoethyl)-tetraacetic acid EIAV equine infectious anaemia virus ELISA enzyme-linked immunosorbent assay EM electron microscope EMA European Medicines Agency EMBL European Molecular Biology Laboratory EMCV Encephalomyocarditis virus EMSA electrophoretic mobility shift assay EMEA European Agency for the Evaluation of Medicinal...
The Cell as the Basic Unit of Life (M. Wink)
Structure and Function of Cellular Macromolecules (M. Wink)
Structure and Functions of a Cell (M. Wink)
Biosynthesis and Function of Macromolecules (DNA, RNA, and Proteins) (M. Wink)
Distributing Proteins in the Cell (Protein Sorting) (M. Wink)
Evolution and Diversity of Organisms (M. Wink)
STANDARD METHODS IN MOLECULAR BIOTECHNOLOGY
Isolation and Purification of Proteins (T. Wieland, M. Lutz)
Peptide and Protein Analysis with Electrospray Tandem Mass Spectrometry (A. Schlosser, W.D. Lehmann)
Isolation of DNA and RNA (H. Weiher, R. Zwacka, I. Herr)
Chromatography and Electrophoresis of Nucleic Acids (H. Weiher, R. Zwacka, I. Herr)
Hybridization of Nucleic Acids (H. Weiher, R. Zwacka, I. Herr)
Use of Enzymes in the Modification of Nucleic Acids (A. Groth, R. Zwacka, H. Weiher, I. Herr)
Polymerase Chain Reaction (A. Mohr, H. Weiher, I. Herr, R. Zwacka)
DNA Sequencing (R. Zwacka, A. Mohr, I. Herr, H. Weiher)
Cloning Procedures (T. Wieland, S. Lutz)
Expression of Recombinant Proteins (T. Wieland, S. Lutz)
Patch Clamp Method (R. Kraft)
Cell Cycle Analysis (S. Wölfl, A. Kitanovic)
Microscopic Techniques (S. Diekmann)
Laser Applications (M. Vogel, R. Fink)
KEY TOPICS
Genomics and Functional Genomics (S.Wiemann, M. Frohme)
Bioinformatics (B. Brors, K. Fellenberg)
Cellular Systems Biology (H. Schmidt-Gienewinkel, S. Legewie, B. Brors, R. König)
Protein?Protein and Protein?DNA Interaction (P. Uetz, E. Pohl)
Drug Research (M. Koegl, R. Tolle, U. Deuschle, C. Kremoser)
Drug Targeting and Prodrugs (G. Fricker)
Molecular Diagnostics in Medicine (S. Wölfl, R. Gessner)
Recombinant Antibodies and Phage Display (S. Dübel)
Transgenic and Gene-Targeted Mice and their Impact in Medical Research (R. Sprengel)
Gene Therapy: Strategies and Vectors (A. Groth, I. Herr)
RNA Interference, Modified DNA, Peptide Nucleic Acid, and Applications in Medicine and Biotechnology (N. Metzler-Nolte, A. Sosniak)
Plant Biotechnology (H. Hillebrand, R. Hell)
Biocatalysis in the Chemical Industry (M. Breuer, B. Hauer)
BIOTECHNOLOGY IN INDUSTRY
Industrial Application: Biotech Industry, Markets, and Opportunities (J. Schüler)
Patents in the Molecular Biotechnology Industry: Legal and Ethical Issues (David B. Resnik)
Drug Approval in the European Union and United States (G. Walsh)
Emergence of a Biotechnology Industry (C. Kremoser)
The 101 of Founding a Biotech Company (C. Kremoser)
Marketing (C. Kremoser)
Further Reading
Abbreviations
Glossary (M. Wink)
Subject Index
Abbreviations
1 Å =0.1 nm aa-tRNA aminoacyl-tRNA AAV adeno-associated virus ABC ATP binding cassette Acetyl-CoA acetyl coenzyme A AcNPV Autographa californica nuclear polyhedrosis virus ACRS amplification-created restriction sites ACTH adrenocorticotropic hormone ADA adenosine deaminase ADEPT antibody-directed enzyme pro-drug therapy ADME-T absorption, distribution, metabolism, excretion and toxicity ADP adenosine diphosphate ADRs adverse drug reactions AEC aminoethylcysteine AFLP amplified fragment length polymorphism AFM atomic force microscope AIDS acquired immune deficiency syndrome ALS amyotrophic lateral sclerosis AMP adenosine monophosphate AMPA α-amino-3-hydroxyl-5-methyl-4-isoxazol-propionate Ampr ampicillin resistance gene AMV avian myeloblastosis virus ANN artificial neural network AO acridine orange AOX1 alcohol oxidase 1 APC anaphase promoting complex ApoB100 apolipoprotein B100 ApoE apolipoprotein E APP amyloid precursor protein ARMS amplification refractory mutation system ARS autonomously replicating sequence ATP adenosine triphosphate att attachment site BAC bacterial artificial chromosome bcl2 B-cell leukemia lymphoma 2 (protein protecting against apoptosis) BfArM German Bundesinstitut für Arzneimittel und Medizinprodukte β-Gal β-galactosidase BHK-21 baby hamster kidney cells BLA biologics licence application BLAST basic local alignment search tool BMP bone morphogenetic proteins bp base pairs BrdU bromodeoxyuridine CA correspondence analysis CAD coronary artery disease CaM-Kinase Ca2+/calmodulin-dependent protein kinase cAMP cyclic AMP cap AAV gene mediating encapsulation CARS coherent anti-Raman scattering CAT Committee for Advanced Therapies CBER Center for Biologics Evaluation and Research CC chromatin remodelling complex CCD charge-coupled device CDER Center for Drug Evaluation and Research CDK cyclin-dependent kinase cDNA copy DNA CDR complementary determining region CDRH Center for Devices and Radiological Health CEO chief executive officer CFP cyan fluorescent protein CFTR cystic fibrosis transmembrane regulator CGAP cancer genome anatomy project CGH comparative genome hybridization CHMP Committee for Medicinal Products for Human Use CHO Chinese hamster ovary CIP calf intestinal phosphatase CML chronic myeloic leukemia CMN Corynebacterium-Mycobacterium-Nocardia group CMV cauliflower mosaic virus CMV Cytomegalovirus CNS central nervous system COMP Committee on Orphan Medicinal Products COS-1 simian cell line, CV-1, transformed by origin-defective mutant of SV40 cpDNA chloroplast DNA CPMV cowpea mosaic virus cPPT-sequence central polypurine tract – regulatory element in lentiviral vectors that facilitates double strand synthesis and the nuclear import of the pre-integration complex CSF colony-stimulating factor CSO contract service organisation CTAB cetyltrimethylammonium bromide CVM Center for Veterinary Medicine CVMP Committee for Medicinal Products for Veterinary Use 2D two-dimensional Da Dalton DAG diacylglycerol DAPI 4,6-diamidino-2-phenylindole dATP deoxyadenosine triphosphate DBD DNA-binding domain DAC divide-and-conquer strategy DD differential display DDBJ DNA Data Bank of Japan ddNTP dideoxynucleotide triphosphate DEAE diethylaminoethyl dHPLC denaturing HPLC DIC differential interference contrast DIP Database of Interacting Proteins DNA deoxyribonucleic acid DNAse deoxyribonuclease dNTP deoxynucleoside triphosphate Dox doxycycline ds diabodies disulfide-stabilized diabodies dsDNA double-stranded DNA dsFv-fragment disulfide-stabilized Fv fragment dsRNA double-stranded RNA DtxR diphtheria toxin repressor Ebola-Z envelope protein of the Ebola-Zaire virus, which has a high affinity to lung epithelial cells EC50 effective concentration, the dose or concentration that produces a 50% effect in the test population within a specified time ECD electron capture dissociation EDTA ethylenediaminetetraacetic acid ee enantiomeric excess EF2 elongation factor 2 EF-Tu elongation factor Tu EGF epidermal growth factor EGFP enhanced green fluorescent protein EGTA ethyleneglycol-bis-(2-aminoethyl)-tetraacetic acid EIAV equine infectious anaemia virus ELISA enzyme-linked immunosorbent assay EM electron microscope EMA European Medicines Agency EMBL European Molecular Biology Laboratory EMCV Encephalomyocarditis virus EMSA electrophoretic mobility shift assay EMEA European Agency for the Evaluation of Medicinal...
