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Applied Biochemistry and Bioengineering, Volume 1: Immobilized Enzyme Principles focuses on the utilization of immobilization techniques for the study and application of enzyme catalysts in a variety of potential end-uses. This book emphasizes the preparation of enzyme-support systems, effects caused by the concurrent phenomena of enzyme-catalyzed reaction kinetics and mass transfer resistances, and how these reactions are incorporated into the design of enzyme-catalyzed reactor systems. The magnitude of the perturbation of the apparent kinetic parameters of an immobilized enzyme that could serve in principle as a measure of the effective concentrations of substrate, modifier, or inhibitor at the site of the enzymic reaction is also explained. This volume is recommended for biological scientists and engineers, as well as researchers interested in the biochemical common denominator that causes the interaction of engineering practice and biological sciences for technological development.
Language
Place of publication
Publishing group
Elsevier Science & Techn.
ISBN-13
978-1-4832-1584-6 (9781483215846)
Schweitzer Classification
List of ContributorsIntroduction to the SeriesPrefaceImmobilized Enzymes-A Survey Text ReferencesThe Chemistry of Enzyme Immobilization I. Introduction II. Immobilization of Enzymes by Adsorption III. Immobilization of Enzymes by Entrapment IV. Immobilization of Enzymes by Covalent Cross-Linking with Bi- or Multifunctional Reagents V. Immobilization of Enzymes by Covalent Coupling to Polymeric Supports VI. Concluding Remarks ReferencesDiffusion and Kinetics with Immobilized Enzymes I. Introduction II. Factors Affecting the Kinetics of Bound Enzymes III. Heterogeneous Enzyme Kinetics IV. Effect of External Diffusional Limitations V. Effect of Internal Diffusional Limitations VI. Analysis of Diffusional Effects and Determination of Kinetic Parameters from Experimental Data VII. Dynamic Effect of Weak Acids and Bases on Heterogeneous Enzyme Kinetics VIII. Electrostatic Effects on Bound-Enzyme Kinetics IX. Coimmobilized Multienzyme Systems X. Perspectives List of Symbols ReferencesDesign and Analysis of Immobilized-Enzyme Flow Reactors I. Introduction II. Enzyme Reactor Types and Their Choice III. Idealized Enzyme Reactor Systems IV. Steady-State Analysis of Mass Transfer and Biochemical Reaction in Enzyme Reactors V. Mass Transfer-Kinetic Models for Specialized Systems: Tubular Reactors with Catalytically Active Wall/Hollow-Fiber Reactors VI. Nonideal Fluid-Flow Patterns in Enzyme Reactors VII. Transient Analysis of Immobilized-Enzyme Reactors VIII. Modeling of Multienzyme Reactor Systems IX. Combined Reaction and Separation Processes X. Process Optimization of Enzyme Reactors XI. Comparison of Relative Efficiencies of Several Reactor Designs XII. Epilog List of Symbols ReferencesIndustrial Applications of Immobilized Enzymes and Immobilized Microbial Cells I. Introduction II. Industrial Applications of Immobilized Enzymes III. Applications of Immobilized Microbial Cells IV. Conclusions ReferencesSubject Index