Practical Methods for Biocatalysis and Biotransformations

Biocatalysts are increasingly used by chemists engaged in finechemical synthesis within both industry and academia. Today, thereexists a huge choice of high-tech enzymes and whole cellbiocatalysts, which add enormously to the repertoire of syntheticpossibilities. Practical Methods for Biocatalysis and Biotransformationsis a "how-to" guide focussing on commercially availableenzymes and strains of microorganisms that are readily obtainedfrom culture collections. The source of starting materials andreagents, hints, tips and safety advice (where appropriate) aregiven to ensure, as far as possible, that the procedures arereproducible. Comparisons to alternative methodology are given andrelevant references to the primary literature are cited. Contentsinclude: * Introduction to the application of biocatalysts in the pharmaindustry * introduction to molecular biological techniques for biocatalystdevelopment * Lipase and protease methods including enzyme supports anddynamic kinetic resolutions * Nitrile hydrolases and hydratases * Alcohol dehydrogenases and co-factor recycling * Epoxide hydrolases * Amino acid synthesis * Whole cell processes * Other biocatalysts, including monoamineoxidases,aminotransterases, and lyases Practical Methods for Biocatalysis and Biotransformationsis an essential text offering validated biocatalyst methods forresearchers and postgraduate students in industry and academia.