Partitioning In Aqueous Two - Phase System

ContributorsPrefaceAbbreviations and Conventions1 History of Aqueous Polymer Two-Phase Partition Text References2 Theoretical Aspects of Partitioning I. Properties of Polymers II. Phase Separation III. Theory of Partitioning References3 Preparation of Phase Systems and Measurement of Their Physicochemical Properties I. Introduction II. Preparation of Phase Systems III. Single-Step Partition Experiments IV. Measurement of Physicochemical Properties of Phase Systems References4 Thin-Layer Countercurrent Distribution and Apparatus I. Introduction II. Principles of Countercurrent Distribution III. Phase System Selection IV. Countercurrent Distribution of Particulate Samples V. Thin-Layer Countercurrent Distribution Apparatus References5 Other Types of Countercurrent Distribution Apparatus and Continuous Flow Chromatography Techniques I. Introduction II. Enhanced Gravity Countercurrent Distribution III. Column Chromatography IV. Countercurrent Chromatography V. Emerging Techniques VI. Commercial Availability References6 Partitioning of Proteins I. Introduction II. Properties Determining the Partitioning Behavior of Proteins III. Practical Procedures Used in Partitioning Proteins IV. Practical Aspects of Protein Extraction V. Multistep Extraction Procedures VI. Analytical Applications VII. Specific Studies References7 Partitioning of Nucleic Acids I. Introductory Remarks II. General Behavior of Nucleic Acids in Aqueous Two-Phase Systems III. Isolation and Fractionation of Nucleic Acids in Aqueous Two-Phase Polymer Systems IV. Fractionation of Chromosomes by Partitioning in Poly(ethylene Glycol)-Dextran Systems V. Applications of Partitioning for Studying Nucleic Acid-Ligand Interactions References8 Interacting Systems and Binding Studied by Partitioning I. Introduction II. Theory III. Examples IV. Conclusion References9 Steroid Receptors and Steroid-Binding Plasma Proteins Studied by Partitioning I. Biological Functions of Steroid-Binding Proteins II. Use of Partitioning for Studying Steroid-Protein Binding III. Use of Partitioning for Studying Molecular Conversions of Glucocorticoid-Receptor Complexes References10 Surface Properties of Cells Reflected by Partitioning: Red Blood Cells as a Model I. Introduction II. What Red Blood Cells Taught Us About Phase Systems III. What Phase Systems Taught Us About Red Blood Cells IV. Comments on Dextran-Ficoll Aqueous Phase Systems V. Conclusion References11 Separation and Subfractionation of Selected Mammalian Cell Populations I. Introduction II. Separation and Subfractionation of Blood Elements III. Partitioning of Bone Marrow Cells: Cells Containing Hemoglobin IV. Fractionation of Lymphoid Cells from Spleen and Other Tissues V. Tissue Cells VI. Tissue Culture Cells VII. Conclusion References12 Partitioning of Bacteria, Virus, and Phage I. Introduction II. Aqueous Two-Phase Partitioning of Bacteria III. Aqueous Two-Phase Partitioning of Virus and Phage IV. Summary References13 Partitioning of Animal Membranes and Organelles I. Introduction II. Relevant Principles in Phase Partitioning as Applied to Cell Particles III. Preparative and Semipreparative Procedures IV. Analytical Phase Partitioning Procedures: Application to Membrane Fragments and Organelles V. Conclusions References14 Partitioning of Plant Cells, Cell Walls, Membranes, and Organelles I. Cells and Cell Walls II. Protoplasts III. Plasma Membranes IV. Intact Chloroplasts V. Leaf Mitochondria VI. Separation of Right-Side-Out and Inside-Out Membrane Vesicles VII. Concluding Remarks References15 Applications of Phase Partitioning in Biotechnology I. Introduction II.