The Protein Protocols Handbook
2nd Edition
Published on 2. October 2007
XXIV, 1146 pages
978-1-59259-169-5 (ISBN)
Description
The Protein Protocols Handbook, Second Edition aims to provide a cross-section of analytical techniques commonly used for proteins and peptides, thus providing a benchtop manual and guide for those who are new to the protein chemistry laboratory and for those more established workers who wish to use a technique for the first time. All chapters are written in the same format as that used in the Methods in Molecular BiologyT series. Each chapter opens with a description of the basic theory behind the method being described. The Materials section lists all the chemicals, reagents, buffers, and other materials necessary for carrying out the protocol. Since the principal goal of the book is to provide experimentalists with a full account of the practical steps necessary for carrying out each protocol successfully, the Methods section contains detailed st- by-step descriptions of every protocol that should result in the successful execution of each method. The Notes section complements the Methods material by indicating how best to deal with any problem or difficulty that may arise when using a given technique, and how to go about making the widest variety of modifications or alterations to the protocol. Since the first edition of this book was published in 1996 there have, of course, been significant developments in the field of protein chemistry.
Reviews / Votes
"the breadth of techniques covered is large and this alone makes the book of practical value. . .The protocols are provided with sufficient detail so as to allow implementation as new methods in any working laboratory. . .It can easily be envisioned that this book would become the subject of a tug of war in a busy laboratory. Most investigators will find value here as will students working in almost any area dealing with proteins. . .This is a compilation not found in other single sources. The very broad scope has considerable practical value. I personally want this in my laboratory."-Doody's Health Sciences Book Review Journal"The wide acceptance of this handbook in the former edition has resulted to this second thoroughly revised and extended second edition considering the recent significant developments in the field of protein chemistry. It is an excellent collection of methods and protocols, giving an essential and compact guide for those new as well as those well established to protein laboratory techniques." - Ernahrungforschung
Review of the first edition:
"The Protein Protocols Handbook has arrived at the right place at the right time."-Trends in Cell Biology
From Reviews of the First Edition
"...a must for researchers in protein chemistry and especially those with less experience..."
-Doody's Health Sciences Book Review Journal
"...a very useful tool for daily work.." -Nahrung-Food
"...definitely an indispensable benchtop handbook...highly recommended for all levels of research within the field." -Bioseparation
"...stands out in an already overcrowded field..."
-Aslib Book Guide
"In his preface the editor wishes the reader luck in his protein analysis, his book represents an invaluable compendium to help achieve this aim." - Molecular Biotechnology
More details
Other editions
Additional editions
John M. Walker
The Protein Protocols Handbook
Book
02/2002
2nd Edition
Humana Press Inc.
€85.59
Article exhausted; check for reprint
John M. Walker
The Protein Protocols Handbook
Book
02/2002
2nd Edition
Humana Press Inc.
€85.59
Article exhausted; check for reprint
Previous edition
John M. Walker
The Protein Protocols Handbook
E-Book
02/2008
1st Edition
Humana
€99.99
Available for download
Content
Quantitation of Proteins.- Electrophoresis of Proteins and Peptides and Detection in Gels.- Blotting and Detection Methods.- Chemical Modification of Proteins and Peptide Production and Purification.- Protein/Peptide Characterization.- Glycoproteins.- Antibody Techniques.- Monoclonal Antibodies.
