Insect Cell Cultures
Fundamental and Applied Aspects
Published on 11. April 2006
VIII, 316 pages
978-0-306-46850-6 (ISBN)
Description
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Reviews / Votes
`In summary, this book covers a wide range of aspects relating to insect cell culture and the baculovirus expression system. It is an authoritative volume which is clearly and conci8sely written by experts selected from industry and academia and is to be highly recommended.'Entomologia Experimentalis et Applicata, 87 (1998)
`This is an authoritative reference text written by leading experts in their respective fields of insects cell culture. The book targets a potential audience ranging from students and researchers in academia through to those concerned in industry with biotechnology applications.'
SGM Quarterly, August (1997)
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Just M. Vlak | Cornelis D. de Gooijer | Johannes Tramper
Insect Cell Cultures
Fundamental and Applied Aspects
Book
08/1996
Kluwer Academic Publishers
€320.99
Shipment within 15-20 days
Content
Insect Cell Lines.- Development and characterization of insect cell lines.- New approaches to insect tissue culture.- Transgenic insect cells: mosquito cell mutants and the dihydrofolate reductase gene.- Insect cell physiology.- Insect cell cultivation: growth and kinetics.- Medium design for insect cell culture.- Viruses.- Baculovirus-insect cell interactions.- Replication patterns and cytopathology of cells infected with baculoviruses.- Construction of baculovirus recombinants.- Passage effect of virus infection in insect cells.- Post-translational modifications in insect cells.- Productivity of insect cells for recombinant proteins.- Chaperone and foldase coexpression in the baculovirus-insect cell expression system.- Engineering Aspects.- Shear sensitivity of insect cells.- Insect cell bioreactors.- Perfusion bioreactors for the production of recombinant proteins in insect cells.- Immobilization of insect cells.- Modelling baculovirus infection of insect cells in culture.- Scale up aspects of sparged insect-cell bioreactors.- Oxygen gradients in small and big sparged insect-cell bioreactors.- Downstream processing of insect cell cultures.- Applications.- Parvovirus diagnostics and vaccine production in insect cells.- Classical swine fever virus diagnostics and vaccine production in insect cells.- Production of multidomain complement glycoproteins in insect cells.- Economic and Regulatory Aspects.- Economics of baculovirus-insect cell production systems.- Safety aspects of insect cell culture.- Regulatory issues in the use of insect-cell culture.
