Vertebrate Phototransduction and the Visual Cycle, Part A

Name: Vertebrate Phototransduction and the Visual Cycle, Part A | Vertebrae Phototransduction and the Visual Cycle : Part A
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Vertebrae Phototransduction and the Visual Cycle : Part A

Academic Press

Published on 1. March 2000

959 pages

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978-0-08-049672-6 (ISBN)

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Front Cover
Vertebrate Phototransduction and the Visual Cycle
Copyright Page
Table of Contents
Contributors to Volume 315
Preface
Volumes in Series
Section I: Expression and Isolation of Opsins
Chapter 1. Heterologous Expression of Bovine Opsin in Pichia pastoris
Chapter 2. Baculovirus Expression System for Expression and Characterization of Functional Recombinant Visual Pigments
Chapter 3. Development of Stable Cell Lines Expressing High Levels of Point Mutants of Human Opsin for Biochemical and Biophysical Studies
Chapter 4. Folding and Assembly of Rhodopsin from Expressed Fragments
Chapter 5. Isolation of Isoelectric Species of Phosphorylated Rhodopsin
Chapter 6. Rhodopsin Trafficking in Photoreceptors Using Retinal Cell-Free System
Section II: Characterization of Opsins
Chapter 7. Preparation and Analysis of Two-Dimensional Crystals of Rhodopsin
Chapter 8. Domain Approach to Three-Dimensional Structure of Rhodopsin Using High Resolution Nuclear Magnetic Resonance
Chapter 9. Analysis of Functional Microdomains in Rhodopsin
Chapter 10. Mapping Tertiary Contacts between Amino Acid Residues within Rhodopsin
Chapter 11. Energetics of Rhodopsin Photobleaching: Photocalorimetric Studies of Energy Storage in Early and Late Intermediates
Chapter 12. Absorption Spectroscopy in Studies of Visual Pigments. Spectral and Kinetic Characterization of Intermediates
Chapter 13. Structural Determinants of Active State Conformation of Rhodopsin. Molecular Biophysics Approaches
Chapter 14. pKa of Protonated Schiff Base of Visual Pigments
Chapter 15. Assays for Detection of Constitutively Active Opsins
Chapter 16. Synthetic Retinals: Convenient Probes of Rhodopsin and Visual Transduction Process
Chapter 17. Assays for Activation of Opsin by all-trans-Retinal
Chapter 18. Assays for Activation of Recombinant Expressed Opsin by all-trans-Retinal
Chapter 19. Electrical Approach to Study Rhodopsin Activation in Single Cells with Early Receptor Current Assay
Chapter 20. Analysis of Amino Acid Residues in Rhodopsin and Cone Visual Pigments That Determine Their Molecular Properties
Chapter 21. Phylogenetic Analysis and Experimental Approaches to Study Color Vision in Vertebrates
Section III: Proteins That Interact with Rhodopsin
Chapter 22. Light Scattering Methods to Monitor Interaction between Rhodopsin-Containing Membranes and Soluble Proteins
Chapter 23. Heterogeneity of Rhodopsin Intermediate State Interacting with Transducin
Chapter 24. Limited Proteolytic Digestion Studies of G Protein Receptor Interaction
Chapter 25. Monitoring Proton Uptake from Aqueous Phase during Rhodopsin Activation
Chapter 26. Use of Peptides-on-Plasmids Combinatorial Library to Identify High Affinity Peptides That Bind Rhodopsin
Chapter 27. Purifiation of Rhodopsin Kinase by Recoverin Affinity Chromatography
Chapter 28. Heterologous Expression and Reconstitution of Rhodopsin with Rhodopsin Kinase and Arrestin
Chapter 29. Arrestin: Mutagenesis, Expression, Purification, and Functional Characterization
Chapter 30. Mapping Interaction Sites between Rhodopsin and Arrestin by Phage Display and Synthetic Peptides
Chapter 31. Characterization of RanBP2-Associated Molecular Components in Neuroretina
Section IV: Transducin and Regulators of G-Protein Signaling
Chapter 32. Intrinsic Biophysical Monitors of Transducin Activation. Fluorescence, UV/Visible Spectroscopy, Light Scattering, and Evanescent Field Techniques
Chapter 33. Fluorescent Probes as Indicators of Conformation Changes in Transducin on Activation
Chapter 34. Gat/Gai1 Chimeras Used to Define Structural Basis of Specific Functions of Gat
Chapter 35. Enzymology of GTPase Acceleration in Phototransduction
Chapter 36. Mutational Analysis of Effectors and Regulators of G-Protein Signaling Interfaces of Transducin
Section V: Photoreceptor Protein Phosphatases
Chapter 37. Isolation and Properties of Protein Phosphatase 2A in Photoreceptors
Chapter 38. Purifiation and Characterization of Protein Phos- phate Type 2C in Photoreceptors
Chapter 39. Photoreceptor Serine/Threonine Protein Phosphatase Type 7: Cloning, Expression, and Functional Analysis
Section VI: Photoreceptor Phosphodiesterase and Guanylyl Cyclase
Chapter 40. Purification and Assay of Bovine Type 6 Photoreceptor Phosphodiesterase and Its Subunits
Chapter 41. Transcriptional Regulation of cGMP Phosphodiesterase B-Subunit Gene
Chapter 42. Inhibition of Photoreceptor cGMP Phosphodiesterase by Its y Subunit
Chapter 43. Kinetics and Regulation of cGMP Binding to Non- catalytic Binding Sites on Photoreceptor Phosphodiesterase
Chapter 44. Purifiation and Autophosphorylation of Retinal Guanylate Cyclase
Chapter 45. Use of Nucleotide a-Phosphorothioates in Studies of Photoreceptor Guanylyl Cyclase: Purification of Guanylyl Cyclase Activating Proteins
Chapter 46. Heterologous Expression and Assays for Photoreceptor Guanylyl Cyclases and Guanylyl Cyclase Activating Proteins
Chapter 47. Spectrophotometric Determination of Retinal Rod Guanylyl Cyclase
Chapter 48. Calcium-Dependent Activation of Membrane Guanylate Cyclase by S100 Proteins
Chapter 49. Characterization of Guanylyl Cyclase and Phosphodiesterase Activities in Single Rod Outer Segments
Section VII: Cyclic Nucleotide-Gated Channels
Chapter 50. Covalent Tethering of Ligands to Retinal Rod Cyclic Nucleotide-Gated Channels: Binding Site Structure and Allosteric Mechanism
Chapter 51. Using State-Specific Modifiers to Study Rod cGMP- Activated Ion Channels Expressed in Xenopus Oocytes
Chapter 52. Identification and Characterization of Calmodulin Binding Sites in cGMP-Gated Channel Using Surface Plasmon Resonance Spectroscopy
Chapter 53. Determination of Fractional Calcium Ion Current in Cyclic Nucleotide-Gated Channels
Chapter 54. Modulation of Rod cGMP-Gated Cation Channel by Calmodulin
Section VIII: Na+ /Ca2+ -K+ Exchanger and ABCR Transporter
Chapter 55. Purification and Biochemical Analysis of cGMP- Gated Channel and Na+ /Ca2+ -K+ Exchanger of Rod Photoreceptors
Chapter 56. Spectrofiuorometric Detection of Na+ /Ca2+ -K+ Exchange
Chapter 57. Purification and Characterization of ABCR from Bovine Rod Outer Segments
Chapter 58. ABCR: Rod Photoreceptor-Specific ABC Transporter Responsible for Stargardt Disease
Author Index
Subject Index

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Vertebrate Phototransduction and the Visual Cycle, Part A

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