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Alcohol and Aldehyde Metabolizing Systems, Volume II: Enzymology and Subcellular Organelles contains some of the papers presented at the Second International Symposium on Alcohol and Aldehyde Metabolizing Systems, held at the University of Pennsylvania in October 1976. Experts in the field from a wide variety of backgrounds tackled the problems of alcohol and aldehyde metabolism, discussed research findings, and examined controversial issues ranging from the molecular structure of enzymes to the clinical actions of alcohol in humans. Comprised of 43 chapters, this volume begins by comparing the enzymatically catalyzed oxidation of glyceraldehyde-3-phosphate and lactate, followed by an analysis of liver alcohol dehydrogenase and its mechanism of action. The reader is methodically introduced to the kinetics and mechanism of activated liver alcohol dehydrogenase; structural and functional changes in different alcohol dehydrogenases during evolution; and localization and properties of aldehyde reductase. Subsequent chapters deal with the correlation between the activity of acetaldehyde dehydrogenase and the structure of mitochondria; the distribution of subcellular organelles; and peroxisomes and microsomes. This book will be of value to practitioners and researchers from a variety of disciplines, including biochemistry, physiology, cell biology, and pharmacology.
Language
Place of publication
Publishing group
Elsevier Science & Techn.
ISBN-13
978-1-4832-6546-9 (9781483265469)
Schweitzer Classification
List of ParticipantsPrefaceEnzyme Structure and Mechanism A Comparison of the Enzymatically Catalyzed Oxidation of Glyceraldehyde-3-Phosphate and Lactate Mechanism of Action of Liver Alcohol Dehydrogenase Extrapolation of Mechanism Studies on Glyceraldehyde-3-Phosphate Dehydrogenase to the Treatment of Avian Muscular DystrophyAlcohol and Aldehyde Dehydrogenase Kinetics and Mechanism of Activated Liver Alcohol Dehydrogenase Solvent Isotope Effects in the Yeast Alcohol Dehydrogenase Reaction Kinetics of Native and Chemically Activated Human Liver Alcohol Dehydrogenase Substrate Specificity and the Hydrophobic Site of Liver Alcohol Dehydrogenase (L-ADH) Properties of Horse Liver Alcohol Dehydrogenase SS Room and Low Temperature Spectral Identification of the Cobalt Bindings Sites in Liver Alcohol Dehydrogenase Heterogeneity in the Rapidly Exchanging Metals of Horse Liver Alcohol Dehydrogenase Magnetic Resonance Studies of the Interactions of Imidazole and Other Ligands with Cobalt Substituted Alcohol Dehydrogenase from Liver Water Proton Relaxation and Spectral Studies of Alcohol Dehydrogenase Partially Substituted with Cobalt Interaction and Activities of NAD+ and NAD+ Analogs with Four Dehydrogenases Modification of Tryptophan in Liver Alcohol Dehydrogenase Structural and Functional Changes in Different Alcohol Dehydrogenases during Evolution Metal Ion Effectors of Horse Liver Aldehyde Dehydrogenases Interaction of Disulfiram with Horse Liver Aldehyde Dehydrogenase Intracellular Location of 3,4-Dihydroxyphenylacetaldehyde and Acetaldehyde Oxidation in Rat Liver Localization and Properties of Aldehyde Reductase Human Liver Aldehyde Dehydrogenase Inhibition of Rat-Liver Aldehyde Dehydrogenases in Vitro and in Vivo by Disulfiram, Cyanamide, and the Alcohol-Sensitizing Compound Coprine Correlation between the Activity of Acetaldehyde Dehydrogenase and the Structure of Mitochondria Induction of Cytosolic Aldehyde Dehydrogenase Isozymes: Influence on Acetaldehyde MetabolismSubcellular Organelles and their Distribution The Plastic and Fluid Nature of the Mitochondrial Energy Transducing Membrane Peroxide Generation in Mitochondria and Utilization by Catalase Metabolism of HjOj and Fatty Acids by Rat Liver Peroxisomes: ImpHcations for Ethanol Detoxification Some Effects of Aging and Lipid-Lowering Drugs on Lipid Metabolism and the Hepatic Endoplasmic ReticulumPeroxisomes and Microsomes On the Question of Whether Cytochrome P-450 Catalyzes Ethanol Oxidation: Studies with Purified Forms of the Cytochrome from Rabbit Liver Microsomes Evidence for the Direct Involvement of Hepatic Cytochrome P450 in Ethanol Metabolism Advantages of Gas Chromatographic Determination of Acetaldehyde in Studies with Ethanol and a Reconstituted Enzyme System Containing Electrophoretically Homogenous Phenobarbital-Inducible Cytochrome P-450 from Rabbit Liver Microsomes Reconstitution of the Hepatic Microsomal Ethanol Oxidizing System (MEOS) in Control Rats and After Ethanol Feeding Microsomal Electron Transport Reactions: The Formation and Utilization of Hydrogen Peroxide as Related to Alcohol Metabolism Studies of the Characterization of the Enzyme Components Participating in the Hepatic-Microsomal Oxidation of Aliphatic Alcohols Quantitation of Pathways Responsible for NADPH-Dependent Methanol, Ethanol, and Butanol Oxidation by Hepatic Microsomes Catalase and Alcohol Dehydrogenase-Mediated Oxidation of Ethanol in Hepatic Microsomes of Acatalasemic Mice Analysis of Mitochondrial and Microsomal Cytochromes in Human Liver Biopsy A Novel Route for the Metabolism of Ethanol: The Oxidation of Ethanol by Hydroxyl Free Radicals Folate Deficiency and Methanol Poisoning in the Rat The Monkey as a Model in Methanol Poisoning Methanol Poisoning: Role of Formate Metabolism in the Monkey Mitochondrial Injury in